Department of Food Science and Technology, Kyungpook National University, Daegu 41566, Korea.
Institute of Agricultural Life Sciences, Dong-A University, Busan 49315, Korea.
Nutrients. 2020 Apr 25;12(5):1205. doi: 10.3390/nu12051205.
Nowadays, new types of vinegar have been developed using various raw materials and biotechnological processes. The fruit of has been extensively distributed in East Asia and used as a folk medication for fatigue. In this study, the vinegar (PV) was produced by a two-step fermentation and was evaluated for its anti-fatigue activity by C2C12 myoblasts and high-intensity exercised rats. The administration of PV significantly improved running endurance and glycogen accumulation in the liver and muscle of PV supplemented rats compared to sedentary and exercised control groups. In addition, PV supplementation elicited lower fatigue-related serum biomarkers, for instance, ammonia, inorganic phosphate, and lactate. PV administered rats exhibited higher lactate dehydrogenase activity and glutathione peroxidase activity, and lower creatine kinase activity and malondialdehyde levels. Furthermore, phenolic compounds in PV were identified using HPLC analysis. The phenolic acids analyzed in PV were protocatechuic acid, syringic acid, chlorogenic acid, and its derivates. These results indicate that the administration of PV with antioxidative property contributes to the improvement of fatigue recovery in exhausted rats. The findings of this study suggest that the PV containing various bioactive constituents can be used as a functional material against fatigue caused by high-intensity exercise.
如今，已经开发出了使用各种原料和生物技术工艺的新型醋。 作为一种民间药物， 已在东亚广泛分布，并用于治疗疲劳。 在这项研究中， 醋（PV）通过两步发酵生产，并通过 C2C12 成肌细胞和高强度运动大鼠评估其抗疲劳活性。 与安静和运动对照组相比，补充 PV 可显著提高大鼠的跑步耐力和肝糖原及肌肉糖原的积累。 此外，补充 PV 还可降低与疲劳相关的血清生物标志物，例如氨、无机磷酸盐和乳酸。 给予 PV 的大鼠表现出更高的乳酸脱氢酶活性和谷胱甘肽过氧化物酶活性，以及更低的肌酸激酶活性和丙二醛水平。 此外，还使用 HPLC 分析鉴定了 PV 中的酚类化合物。 在 PV 中分析的酚酸为原儿茶酸、丁香酸、绿原酸及其衍生物。 这些结果表明，具有抗氧化特性的 PV 的给药有助于改善疲劳大鼠的疲劳恢复。 这项研究的结果表明，含有各种生物活性成分的 PV 可作为对抗高强度运动引起的疲劳的功能性物质。
