Granström M, Wretlind B, Markman B, Cryz S
Department of Vaccine Production, National Bacteriological Laboratory, Stockholm, Sweden.
J Clin Microbiol. 1988 Nov;26(11):2257-61. doi: 10.1128/jcm.26.11.2257-2261.1988.
A highly sensitive enzyme-linked immunosorbent assay was developed for Klebsiella capsular polysaccharide (CPS) and used to evaluate the immunoglobulin G (IgG) antibody response to a 24-valent CPS vaccine in seven adult volunteers. The median rise in titer to all vaccine antigens in samples from the volunteers was significant (twofold or greater). Significant IgG responses to 11 immunologically related serotypes not included in the vaccine were also noted. The mean cross-reacting IgG titer of 127.2 was only slightly lower than the mean titer of 175.7 to the serotypes in the vaccine (P less than 0.05). The mean 29.9-fold increase in titer to the serotypes in the vaccine was significantly higher than the mean 13.5-fold increase in titer to the additional antigens (P less than 0.001). The difference was partly because of the significantly lower (P less than 0.01) natural antibody titers in the preimmune sera to the serotypes in the vaccine, compared with those to serotypes not included in the vaccine. The selection of vaccine serotypes was based on the frequency of serotype isolation from cases of Klebsiella bacteremia. The above findings, which show low levels of natural antibody to these serotypes, support the hypothesis that anti-CPS antibody is protective against bacteremic disease.
开发了一种用于检测肺炎克雷伯菌荚膜多糖（CPS）的高灵敏度酶联免疫吸附测定法，并用于评估7名成年志愿者对24价CPS疫苗的免疫球蛋白G（IgG）抗体反应。志愿者样本中针对所有疫苗抗原的滴度中位数升高显著（两倍或更高）。还注意到对疫苗中未包含的11种免疫相关血清型有显著的IgG反应。127.2的平均交叉反应IgG滴度仅略低于针对疫苗中血清型的175.7的平均滴度（P小于0.05）。针对疫苗中血清型的滴度平均增加29.9倍显著高于针对其他抗原的滴度平均增加13.5倍（P小于0.001）。这种差异部分是因为与针对未包含在疫苗中的血清型相比，免疫前血清中针对疫苗中血清型的天然抗体滴度显著更低（P小于0.01）。疫苗血清型的选择基于从肺炎克雷伯菌血症病例中分离血清型的频率。上述显示对这些血清型天然抗体水平较低的发现支持了抗CPS抗体对菌血症性疾病具有保护作用的假说。
