Tseng Yi-Kai, Chen Yu-Chia, Hou Chien-Jui, Deng Fu-Sheng, Liang Shen-Huan, Hoo Sin Yong, Hsu Chih-Chieh, Ke Cai-Ling, Lin Ching-Hsuan
Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, Taipei City 100-116, Taiwan.
Department of Molecular Microbiology and Immunology, Brown University, Providence, RI 02912, USA.
Microorganisms. 2020 May 1;8(5):660. doi: 10.3390/microorganisms8050660.
Molecular mechanisms of biofilm formation in and current methods for biofilm analyses in this fungal pathogen are limited. (2) Methods: Biofilm biomass and crystal violet staining of the wild-type and each gene mutant strain of were evaluated on silicone under synthetic urine culture conditions. (3) Results: Seven media were tested to compare the effects on biofilm growth with or without silicone. Results showed that biofilm cells of were unable to form firm biofilms on the bottom of 12-well polystyrene plates. However, on a silicone-based platform, Roswell Park Memorial Institute 1640 (RPMI 1640), yeast nitrogen base (YNB) + 1% glucose, and synthetic urine media were able to induce strong biofilm growth. In particular, replacement of Spider medium with synthetic urine in the adherence step and the developmental stage is necessary to gain remarkably increased biofilms. Interestingly, unlike , the deletion strain but not the other five biofilm-associated mutants did not cause a significant reduction in biofilm formation, suggesting that the biofilm regulatory circuits of the two species are divergent. (4) Conclusions: This system for biofilm analyses will become a useful tool to unveil the biofilm regulatory network in .
该真菌病原体中生物膜形成的分子机制以及目前用于生物膜分析的方法有限。（2）方法：在合成尿液培养条件下，在硅胶上评估野生型及各基因缺失突变株的生物膜生物量和结晶紫染色情况。（3）结果：测试了七种培养基，以比较有无硅胶时对生物膜生长的影响。结果显示，该菌的生物膜细胞无法在12孔聚苯乙烯板底部形成牢固的生物膜。然而，在基于硅胶的平台上，罗斯威尔公园纪念研究所1640培养基（RPMI 1640）、酵母氮源培养基（YNB）+1%葡萄糖以及合成尿液培养基能够诱导强烈的生物膜生长。特别是，在黏附步骤和发育阶段用合成尿液替代Spider培养基对于显著增加生物膜形成是必要的。有趣的是，与该菌不同，该菌的缺失突变株而非其他五个与生物膜相关的突变株并未导致生物膜形成显著减少，这表明这两个物种的生物膜调控回路存在差异。（4）结论：该用于该菌生物膜分析的系统将成为揭示该菌生物膜调控网络的有用工具。
