University of Manitoba, Department of Medical Microbiology, Winnipeg Manitoba, Canada.
Special Bacteriology Unit, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg Manitoba, Canada.
Int J Syst Evol Microbiol. 2020 May;70(5):3534-3540. doi: 10.1099/ijsem.0.004153.
Seven strains of an unidentifiable species recovered from blood cultures, urine or cerebrospinal fluid over 26 years, closest to but differentiated from by 16S rRNA gene and partial gene sequencing, were studied. In November 2017, Atasayar described a blood culture isolate as sp. nov., which had >99 % similarity by 16S rRNA gene sequencing to the Canadian strains. In January 2018, Jani described sp. nov., recovered from the Godavari River, India, which also had >99 % similarity by 16S/ sequencing to the Canadian strains and In May 2018, Wei described recovered from hadopelagic water; this too had >99 % similarity by 16S rRNA gene sequencing to , and the Canadian strains. DSM 103494 and LMG 29598 were acquired and whole genome sequencing was performed (not previously done). Results were compared with genomes from (GenBank accession NQMQ01) and the Canadian isolates. We found that these ten genomes formed a single taxon when compared using digital DNA-DNAhybridization, average nucleotide identity using blastn and average amino acid identity criteria but exhibited some subtle biochemical and chemotaxonomic differences. Heuristically, we propose that and are later heterotypic synonyms of, and the Canadian isolates are identifiable as, . We provide an emended description of Atasayar . 2017; genomes ranged from 2.48 to 2.69 Mb ( DSM 103494, 2.62 Mb) with G+C content of 65.1-65.6 mol% (WGS), recovered from clinical and environmental sites.
26 年来,从血液培养物、尿液或脑脊液中分离出的七种无法识别的 种菌株,通过 16S rRNA 基因和部分 基因测序与最接近但有区别的 种进行了研究。2017 年 11 月,Atasayar 描述了一种血培养分离株为 sp. nov.,16S rRNA 基因测序与加拿大菌株的相似度>99%。2018 年 1 月,Jani 描述了一种从印度戈达瓦里河分离出的 sp. nov.,16S/测序与加拿大菌株的相似度也>99%,2018 年 5 月,Wei 描述了从海洋水层分离出的 sp. nov.,16S rRNA 基因测序与 、 和加拿大菌株的相似度也>99%。DSM 103494 和 LMG 29598 被获得并进行了全基因组测序(以前没有做过)。结果与 (GenBank 登录号 NQMQ01) 和加拿大分离株的基因组进行了比较。我们发现,当使用数字 DNA-DNA 杂交、blastn 平均核苷酸同一性和平均氨基酸同一性标准比较这些十个基因组时,它们形成了一个单一的分类群,但表现出一些微妙的生化和化学分类学差异。我们推测 和 是 的后来的异型同义词,而加拿大分离株可鉴定为 。我们提供了 Atasayar 的修正描述。2017 年;基因组大小为 2.48-2.69 Mb(DSM 103494,2.62 Mb),G+C 含量为 65.1-65.6 mol%(WGS),从临床和环境样本中分离得到。