Shandong Provincial Key Laboratory of Plant Stress, College of life Sciences, Shandong Normal University, Jinan, Shandong 250014, China.
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Trends Plant Sci. 2020 Sep;25(9):859-867. doi: 10.1016/j.tplants.2020.04.005. Epub 2020 May 3.
N-methyladenosine (mA) is the most common type of eukaryotic mRNA modification. It plays an important role in regulating plant growth and development and stress resistance. mA modification influences nearly all aspects of RNA metabolism and functionality and has great potential for improving crop quality. However, changing mA modification levels as a whole may have unpredictable effects, making it impossible to accurately predict the effect of specific mA modifications on RNA. In this opinion article, the main challenges and possible solutions for exploring mA modification functions in plant systems are discussed. An mA editing platform that uses new high-throughput methods to identify mA modification at single-base resolution, and genome editing for selective editing of specific mA sites for crop improvement is proposed.
N6-甲基腺嘌呤（m6A）是真核生物 mRNA 修饰中最常见的类型。它在调控植物生长发育和抗逆性方面发挥着重要作用。m6A 修饰几乎影响 RNA 代谢和功能的所有方面，在提高作物品质方面具有巨大的潜力。然而，整体改变 m6A 修饰水平可能会产生不可预测的影响，使得无法准确预测特定 m6A 修饰对 RNA 的影响。在这篇观点文章中，讨论了在植物系统中探索 m6A 修饰功能的主要挑战和可能的解决方案。提出了一种 m6A 编辑平台，该平台使用新的高通量方法以单碱基分辨率识别 m6A 修饰，并进行基因组编辑以选择性编辑特定的 m6A 位点，从而改良作物。
