School of Dentistry, The University of Utah, Salt Lake City, Utah.
Department of Internal Medicine, Division of Epidemiology, The University of Utah, Salt Lake City, Utah.
J Histochem Cytochem. 2020 May;68(5):305-318. doi: 10.1369/0022155420922948.
Our previous studies indicated that YIGSR-A99 peptides chemically conjugated to fibrin hydrogel (FH) and applied to wounded submandibular gland (SMG) in vivo, formed new organized salivary tissue, whereas wounded SMG treated with FH alone or in the absence of a scaffold showed disorganized collagen formation and poor tissue healing. While these studies indicated that damaged SMG grow and differentiate when treated with FH containing L1 peptide, they were performed only in female mice. However, there is a well-established sexual dimorphism present in mouse SMG (e.g., males develop well-differentiated granular convoluted tubules, but these structures are poorly developed in females) and little is known about how these sex differences influence wound healing events. Therefore, the goal of this study was to conduct comparative analyses of regeneration patterns in male and female mice using L-FH in a wounded SMG mouse model. Particularly, we focused on sex-dependent wound healing events such as macrophage polarization, vascularization, tissue organization, and collagen deposition, and how these events affect salivary gland functioning.
我们之前的研究表明，将 YIGSR-A99 肽化学偶联到纤维蛋白水凝胶 (FH) 上，并应用于体内受伤的颌下腺 (SMG)，可形成新的有组织的唾液组织，而单独用 FH 或没有支架处理的受伤 SMG 则表现出胶原形成紊乱和组织愈合不良。虽然这些研究表明，用含有 L1 肽的 FH 处理受损的 SMG 会生长和分化，但这些研究仅在雌性小鼠中进行。然而，在小鼠 SMG 中存在着明显的性别二态性（例如，雄性小鼠发育出成熟的颗粒状卷曲小管，但这些结构在雌性小鼠中发育不良），而且对于这些性别差异如何影响伤口愈合事件知之甚少。因此，本研究的目的是使用 L-FH 在受伤的 SMG 小鼠模型中对雄性和雌性小鼠的再生模式进行比较分析。特别是，我们关注了巨噬细胞极化、血管生成、组织组织和胶原沉积等依赖于性别的伤口愈合事件，以及这些事件如何影响唾液腺的功能。
