Popescu Roua Gabriela, Voicu Sorina Nicoleta, Pircalabioru Gratiela Gradisteanu, Ciceu Alina, Gharbia Sami, Hermenean Anca, Georgescu Sergiu Emil, Panaite Tatiana Dumitra, Dinischiotu Anca
Department of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, Romania.
Department of Pharmacy, Faculty of Pharmacy, Titu Maiorescu University, 004051 Bucharest, Romania.
Animals (Basel). 2020 May 9;10(5):823. doi: 10.3390/ani10050823.
The purpose of this study was to examine the effects of dietary inclusion of two additives at the final concentration of 0.5% bilberry (E1) and 1% walnut (E2) leaves powder in the basal diet on digestive health of hens. A total number of 90 Tetra SL hens were divided into two experimental groups (E1 and E2) and one control group (C) consisting of 30 hens each. After four weeks, 10 hens of each group were sacrificed and tissue samples and intestinal content were taken from the duodenum, jejunum, and cecum in order to perform histological, enzymatic, and microbiota analyses. In groups E1 and E2, the histological analysis showed a significant increase of villus height, resulting probably in increased absorption of nutrients in duodenum and jejunum. A decrease in the specific activity of alpha-amylase and trypsin in E1 and E2 for both duodenum and jejunum compared to the control one was also recorded. In addition, the maltase and invertase specific activity in duodenum increased, a tendency that was kept for maltase but not for invertase in jejunum. The cecal microbiota of E1 and E2 individuals was characterized by an increase of and and a decrease of . In conclusion, our results indicate that bilberry and walnut leaves additives in feed may improve the health status of the poultry gastrointestinal tract.
本研究的目的是检验在基础日粮中添加两种添加剂（最终浓度为0.5%的越橘（E1）和1%的核桃（E2）叶粉）对母鸡消化健康的影响。总共90只特特拉SL母鸡被分为两个实验组（E1和E2）和一个对照组（C），每组各30只母鸡。四周后，每组宰杀10只母鸡，并从十二指肠、空肠和盲肠采集组织样本和肠内容物，以进行组织学、酶学和微生物群分析。在E1和E2组中，组织学分析显示绒毛高度显著增加，这可能导致十二指肠和空肠中营养物质的吸收增加。与对照组相比，E1和E2组十二指肠和空肠中的α-淀粉酶和胰蛋白酶的比活性也有所降低。此外，十二指肠中的麦芽糖酶和蔗糖酶比活性增加，空肠中的麦芽糖酶保持了这种趋势，但蔗糖酶没有。E1和E2组个体的盲肠微生物群的特点是 和 增加， 减少。总之，我们的结果表明，饲料中的越橘和核桃叶添加剂可能会改善家禽胃肠道的健康状况。
