Department of Biochemistry, University of Washington, USA.
Department of Biochemistry, University of Washington, USA.
Curr Opin Cell Biol. 2020 Oct;66:28-33. doi: 10.1016/j.ceb.2020.04.006. Epub 2020 May 14.
Compartmentalization of metabolic enzymes through protein-protein interactions is an emerging mechanism for localizing and regulating metabolic activity. Self-assembly into linear filaments is a common strategy for cellular compartmentalization of enzymes. Polymerization is often driven by changes in the metabolic state of the cell, suggesting that it is a strategy for shifting metabolic flux in response to cellular demand. Although polymerization of metabolic enzymes is widespread, observed from bacteria to humans, we are just beginning to appreciate their role in regulating cellular metabolism. In most cases, one functional role of metabolic enzyme filaments is allosteric control of enzyme activity. Here, we highlight recent findings, providing insight into the structural and functional significance of filamentation of metabolic enzymes in cells.
通过蛋白质-蛋白质相互作用将代谢酶分隔开是一种新兴的定位和调节代谢活性的机制。自我组装成线性纤维是细胞内酶分隔的常见策略。聚合通常由细胞代谢状态的变化驱动，这表明它是一种根据细胞需求转移代谢通量的策略。尽管代谢酶的聚合很普遍，从细菌到人类都有观察到，但我们才刚刚开始了解它们在调节细胞代谢中的作用。在大多数情况下，代谢酶纤维的一个功能作用是酶活性的变构控制。在这里，我们重点介绍了最近的发现，深入了解了代谢酶在细胞中纤维形成的结构和功能意义。
