Ocean College, Zhoushan Campus, Zhejiang University, Zhoushan 316021, China.
College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Mar Drugs. 2020 May 14;18(5):258. doi: 10.3390/md18050258.
Mariana Trench sediments are enriched in microorganisms, however, the structures and bioactivities of their secondary metabolites are not very known. In this study, a fungus sp. SY2107 was isolated from a sample of Mariana Trench sediment collected at a depth of 11000 m and an extract prepared from the culture of this fungus in rice medium showed antimicrobial activities. Chemical investigation on this active extract led to the isolation of 16 compounds, including one novel meroterpenoid, named andrastone C. Structure of the new compound was elucidated based on high-resolution electrospray ionization mass spectroscopy (HRESIMS) data, extensive nuclear magnetic resonance (NMR) spectroscopic analyses and a single crystal X-ray diffraction. The crystal structure of a known meroterpenoid andrastone B was also reported in this study. Both andrastones B and C exhibited antimicrobial activities against methicillin-resistant Staphylococcus aureus (MRSA) Escherichia coli, and Candida albicans with minimum inhibitory concentration (MIC) values in a range from 6 to 13 g/mL.
马里亚纳海沟沉积物中富含微生物，但它们的次生代谢产物的结构和生物活性并不为人所知。在这项研究中，从马里亚纳海沟沉积物中深度为 11000 米的样本中分离出一株真菌 sp. SY2107，从该真菌在大米培养基中的培养物中制备的提取物显示出抗菌活性。对这种活性提取物的化学研究导致分离出 16 种化合物，包括一种新型的混合萜类化合物，命名为安特拉石 C。根据高分辨率电喷雾电离质谱（HRESIMS）数据、广泛的核磁共振（NMR）光谱分析和单晶 X 射线衍射，确定了新化合物的结构。本研究还报道了一种已知的混合萜类化合物安特拉石 B 的晶体结构。安特拉石 B 和 C 均表现出抗甲氧西林耐药金黄色葡萄球菌（MRSA）、大肠杆菌和白色念珠菌的抗菌活性，最小抑菌浓度（MIC）值在 6 至 13 g/mL 范围内。
