Dos Santos Tatiane Souza, Teng Po-Yun, Yadav Sudhir, Castro Fernanda Lima de Souza, Gould Rebecca Lizabeth, Craig Steven Wesley, Chen Chongxiao, Fuller Alberta Lorraine, Pazdro Robert, Sartori José Roberto, Kim Woo Kyun
Department of Breeding and Animal Nutrition, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, Brazil.
Department of Poultry Science, University of Georgia (UGA), Athens, GA, United States.
Front Vet Sci. 2020 Apr 28;7:230. doi: 10.3389/fvets.2020.00230. eCollection 2020.
An experiment was conducted to evaluate the effect of different levels of inorganic copper and zinc on growth performance, intestinal permeability, intestinal lesion scores, oocyst shedding, antioxidant properties and bone quality in broilers challenged with spp. A total of 360 d-old male Cobb broiler chickens were housed in floor cages for 12 days at the Poultry Research Center. At 12 days of age, birds were placed in grower Petersime batteries and distributed in a completely randomized design with 10 birds per cage, six replicates per treatment, and six treatments. There were six corn-soybean meal-based dietary treatments: non-challenged control (NC), challenged control (CC), 100 ppm Cu (100 Cu), 150 ppm Cu (150 Cu), 80 ppm Zn (80 Zn), and 100 ppm Zn (100 Zn). Broilers received the treatment diets for 9 days (12-20d). Birds, except NC, were challenged with (50,000 oocysts/bird), (50,000 oocysts/bird), and (250,000 oocysts/bird) on 14d. On 20d, the growth performance was recorded, and one bird/cage was used for analysis of intestinal permeability, antioxidant properties and bone quality. Lesion score was recorded at 20 days of age in eight birds/cage. The means were subjected to ANOVA and, when significant, compared by Duncan's test. Intestinal permeability was significantly improved when birds received the 100 Zn diet ( < 0.05). In addition, lesion scores on duodenum were reduced when broilers received diets 150 Cu as compared to CC diet ( < 0.05). However, growth performance was not positively influenced by inclusion of inorganic minerals as compared to the NC diet. Furthermore, activity of superoxide dismutase and bone quality were not affected, whereas glutathione status was improved with mineral supplementation in all groups. This study showed that Cu and Zn supplementation improves intestinal integrity during the spp. infection, suggesting that Cu and Zn supplementation would be a potential strategy to reduce detrimental effects of infection in broilers.
进行了一项实验，以评估不同水平的无机铜和锌对感染球虫的肉鸡生长性能、肠道通透性、肠道损伤评分、卵囊排出、抗氧化性能和骨骼质量的影响。总共360只1日龄雄性科宝肉鸡在禽类研究中心的地面笼中饲养12天。12日龄时，将鸡放入生长鸡彼得西姆笼中，并按照完全随机设计进行分配，每笼10只鸡，每个处理6个重复，共6个处理。有六种以玉米-豆粕为基础的日粮处理：未感染对照（NC）、感染对照（CC）、100 ppm铜（100 Cu）、150 ppm铜（150 Cu）、80 ppm锌（80 Zn）和100 ppm锌（100 Zn）。肉鸡接受处理日粮9天（12 - 20日龄）。除NC组外，在14日龄时，鸡用柔嫩艾美耳球虫（50,000个卵囊/只）、毒害艾美耳球虫（50,000个卵囊/只）和巨型艾美耳球虫（250,000个卵囊/只）进行感染。在20日龄时，记录生长性能，每个笼子选一只鸡用于分析肠道通透性、抗氧化性能和骨骼质量。在20日龄时记录每个笼子8只鸡的损伤评分。对平均值进行方差分析，当有显著差异时，通过邓肯氏检验进行比较。当鸡采食100 Zn日粮时，肠道通透性显著改善（P < 0.05）。此外，与CC日粮相比，肉鸡采食150 Cu日粮时十二指肠的损伤评分降低（P < 0.05）。然而，与NC日粮相比，添加无机矿物质对生长性能没有产生积极影响。此外，超氧化物歧化酶活性和骨骼质量未受影响，而所有组添加矿物质后谷胱甘肽状态得到改善。本研究表明，在感染球虫期间补充铜和锌可改善肠道完整性，这表明补充铜和锌可能是减少肉鸡球虫感染有害影响的一种潜在策略。
