Ihnatouski Mikhail, Pauk Jolanta, Karev Dmitrij, Karev Boris
Scientific and Research Department, Yanka Kupala State University of Grodno, Grodno, Ozheshko str., 22, 230023 Grodno, Belarus.
Biomedical Engineering Institute, Bialystok University of Technology, Wiejska 45A, 15-351 Bialystok, Poland.
Materials (Basel). 2020 May 16;13(10):2302. doi: 10.3390/ma13102302.
In osteoarthrosis, pathological features of articular cartilage are associated with degeneration and nanomechanical changes. The aim of this paper is to show that indentation-atomic force microscopy can monitor wear-related biomechanical changes in the hip joint of patients with osteoarthritis. Fifty patients (N = 50), aged 40 to 65, were included in the study. The mechanical properties and the submicron surface morphology of hyaline cartilage were investigated using atomic force microscopy. Measurements of the roughness parameters of cartilage surfaces were performed, including the arithmetic average of absolute values (), the maximum peak height (), and the mean spacing between local peaks (). The arithmetic mean of the absolute values of the height of healthy cartilage was 86 nm, while wear began at = 73 nm. The maximum changes of values of the roughness parameters differed from the healthy ones by 71%, 80%, and 51% for , , and , respectively. Young's modulus for healthy cartilage surfaces ranged from 1.7 to 0.5 MPa. For the three stages of cartilage wear, Young's modulus increased, and then it approached the maximum value and decreased. AFM seems to be a powerful tool for surface analysis of biological samples as it enables indentation measurements in addition to imaging.
在骨关节炎中，关节软骨的病理特征与退变及纳米力学变化相关。本文旨在表明压痕原子力显微镜可监测骨关节炎患者髋关节中与磨损相关的生物力学变化。本研究纳入了50例年龄在40至65岁之间的患者（N = 50）。使用原子力显微镜研究了透明软骨的力学性能和亚微米表面形态。对软骨表面的粗糙度参数进行了测量，包括绝对值的算术平均值（）、最大峰高（）和局部峰之间的平均间距（）。健康软骨高度绝对值的算术平均值为86 nm，而磨损始于 = 73 nm时。粗糙度参数值的最大变化分别比健康软骨的相应参数值在、和方面高出71%、80%和51%。健康软骨表面的杨氏模量范围为1.7至0.5 MPa。对于软骨磨损的三个阶段，杨氏模量先升高，然后接近最大值并下降。原子力显微镜似乎是生物样品表面分析的有力工具，因为它除了成像外还能进行压痕测量。
