Dai Yu-Lin, Kim Eun-A, Luo Hao-Ming, Jiang Yun-Fei, Oh Jae-Young, Heo Soo-Jin, Jeon You-Jin
1Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 130117 China.
2Department of Marine Life Science, Jeju National University, Jeju, 63243 Republic of Korea.
J Food Sci Technol. 2020 Jun;57(6):2283-2292. doi: 10.1007/s13197-020-04266-z. Epub 2020 Feb 20.
In this study, the saponin-rich fractions of five individual (two Red and three Black) sea cucumbers () in South Korea were investigated for their antiproliferative effect against HL-60, B16F10, MCF-7, and Hep3B tumor cell lines. The red sea cucumber saponin-rich fraction (SSC) from Jeju Island (JRe) decreased the growth of HL-60 with an IC value of 23.55 ± 3.40 μg/mL, which represented the strongest anticancer activity among the extracts. Further, SSC downregulated B-cell lymphoma extra-large (Bcl-xL), while upregulating, to different degrees, Bcl-2-associated X protein (Bax), caspase-9, caspase-3, PARP cleavage, and apoptotic bodies in cancer cells. Evidence for SSC inducing apoptosis via the mitochondria-mediated pathway was found. The contents of SSCs were determined using ultra high-performance liquid chromatography coupled with a quadrupole orbitrap mass spectrometry to comparatively evaluate the regional influence. In West Sea, the total SSC content of was 15.5 mg/g, representing the highest content, while in the South Sea yielded the lowest content at 8 mg/g. The major saponin constituent in SSC was identified as Holotoxin A, which may the anti-tumor compound in .
在本研究中，对韩国的五种个体海参（两种红色和三种黑色）中富含皂苷的部分进行了研究，以考察其对HL-60、B16F10、MCF-7和Hep3B肿瘤细胞系的抗增殖作用。来自济州岛（JRe）的红色海参富含皂苷的部分（SSC）使HL-60的生长降低，IC值为23.55±3.40μg/mL，这代表提取物中最强的抗癌活性。此外，SSC下调了B细胞淋巴瘤-超大蛋白（Bcl-xL），同时不同程度地上调了癌细胞中的Bcl-2相关X蛋白（Bax）、半胱天冬酶-9、半胱天冬酶-3、聚（ADP-核糖）聚合酶裂解产物和凋亡小体。发现了SSC通过线粒体介导的途径诱导细胞凋亡的证据。使用超高效液相色谱-四极杆轨道阱质谱联用技术测定SSC的含量，以比较评估区域影响。在西海，[海参名称未给出]的总SSC含量为15.5mg/g，为最高含量，而在南海的[海参名称未给出]含量最低，为8mg/g。SSC中的主要皂苷成分被鉴定为全毒素A，它可能是[海参名称未给出]中的抗肿瘤化合物。
