Leśniak-Ziółkowska Katarzyna, Śmiga-Matuszowicz Monika, Blacha-Grzechnik Agata, Student Sebastian, Brzychczy-Włoch Monika, Krok-Borkowicz Małgorzata, Pamuła Elżbieta, Simka Wojciech, Kazek-Kęsik Alicja
Faculty of Chemistry, Silesian University of Technology, B. Krzywoustego Street 6, 44-100, Gliwice, Poland.
Faculty of Chemistry, Silesian University of Technology, M. Strzody 9 Street, 44-100, Gliwice, Poland.
Bioact Mater. 2020 May 24;5(3):709-720. doi: 10.1016/j.bioactmat.2020.04.020. eCollection 2020 Sep.
This paper describes a formation of hybrid coatings on a Ti-2Ta-3Zr-36Nb surface. This is accomplished by plasma electrolytic oxidation and a dip-coating technique with poly(adipic anhydride) ((CHO)n) that is loaded with drugs: amoxicillin (CHNOS), cefazolin (CHNOS) or vancomycin (CHClNO · xHCl). The characteristic microstructure of the polymer was evaluated using scanning electron microscopy and confocal microscopy. Depending on the surface treatment, the surface roughness varied (between 1.53 μm and 2.06 μm), and the wettability was change with the over of time. X-ray photoelectron spectroscopy analysis showed that the oxide layer did not affect the polymer layer or loaded drugs. However, the drugs lose their stability in a phosphate-buffered saline solution after 6.5 h of exposure, and its decrease was greater than 7% (HPLC analysis). The stability, drug release and concentration of the drug loaded into the material were precisely analyzed by high-performance liquid chromatography. The results correlated with the degradation of the polymer in which the addition of drugs caused the percent of degraded polymer to be between 35.5% and 49.4% after 1 h of material immersion, depending on the mass of the loaded drug and various biological responses that were obtained. However, all of the coatings were cytocompatible with MG-63 osteoblast-like cells. The drug concentrations released from the coatings were sufficient to inhibit adhesion of reference and clinical bacterial strains (). The coatings with amoxicillin showed the best results in the bacterial inhibition zone, whereas coatings with cefazolin inhibited adhesion of the above bacteria on the surface.
本文描述了在Ti-2Ta-3Zr-36Nb表面形成混合涂层的过程。这是通过等离子体电解氧化和用负载药物的聚(己二酸酐)((CHO)n)进行浸涂技术来实现的,这些药物包括阿莫西林(CHNOS)、头孢唑林(CHNOS)或万古霉素(CHClNO·xHCl)。使用扫描电子显微镜和共聚焦显微镜对聚合物的特征微观结构进行了评估。根据表面处理的不同,表面粗糙度有所变化(在1.53μm至2.06μm之间),并且润湿性会随时间而改变。X射线光电子能谱分析表明,氧化层不会影响聚合物层或负载的药物。然而,在暴露于磷酸盐缓冲盐溶液6.5小时后,药物失去了稳定性,其下降幅度大于7%(高效液相色谱分析)。通过高效液相色谱精确分析了负载到材料中的药物的稳定性、药物释放和浓度。结果与聚合物的降解相关,其中根据负载药物的质量和所获得的各种生物学反应,在材料浸入1小时后,药物的添加导致降解聚合物的百分比在35.5%至49.4%之间。然而,所有涂层都与MG-63成骨样细胞具有细胞相容性。从涂层中释放的药物浓度足以抑制参考和临床细菌菌株的粘附()。含阿莫西林的涂层在细菌抑制区显示出最佳结果,而含头孢唑林的涂层抑制了上述细菌在表面的粘附。