State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Science and Technology of China, Hefei 230026, PR China.
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
Mater Sci Eng C Mater Biol Appl. 2020 Aug;113:110936. doi: 10.1016/j.msec.2020.110936. Epub 2020 Apr 21.
Pathogenic bacterial infections associated with wound healing progress usually result in serious complications. Herein, biocompatible and antimicrobial electrospun nanofibrous mats with photodynamic therapy (PDT) effect were fabricated to accelerate the infected wound healing. The nanofibrous mats were fabricated by co-electrospining of polyanionic poly(γ-glutamic acid) (γ-PGA) and cationic photosensitizer 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetra (p-toluenesulfonate) (TMPyP) in aqueous solution and stabilized by the chemical crosslinking. The as-prepared nanofibrous mats can not only confer the moist microenvironment to the wound bed, but also provide potent bactericidal activity upon visible light irradiation by releasing the cytotoxic reactive oxygen species (ROS). The antibacterial assay in vitro showed that they can effectively eradicate the board-spectrum bacteria at a relatively low loading dose of TMPyP (e.g., 0.1 wt%). Meanwhile, those nanofibrous mats showed good biocompatibility with no obvious adverse effects on mammalian cells and red blood cells (RBCs). The animal test in vivo suggested that the restrained inflammatory reaction and better wound healing could be achieved upon timely and effective antibacterial treatment with negligible local toxicities. This biocompatible and antibacterial γ-PGA-TMPyP nanofibrous mat may show great potential in practical infection-resistant applications, particularly for wound dressing applications.
与伤口愈合进展相关的致病细菌感染通常会导致严重的并发症。在此，制备了具有光动力疗法（PDT）效果的生物相容性和抗菌性电纺纳米纤维垫，以加速感染伤口的愈合。纳米纤维垫是通过在水溶液中共电纺聚阴离子聚（γ-谷氨酸）（γ-PGA）和阳离子光敏剂 5,10,15,20-四（1-甲基吡啶-4-基）卟啉四（对甲苯磺酸盐）（TMPyP）制备的，并通过化学交联进行稳定。所制备的纳米纤维垫不仅可以为伤口床提供湿润的微环境，而且在可见光照射下通过释放细胞毒性活性氧物质（ROS）还可以提供强大的杀菌活性。体外抗菌试验表明，它们可以在相对较低的 TMPyP 负载量（例如 0.1wt%）下有效消除广谱细菌。同时，这些纳米纤维垫对哺乳动物细胞和红细胞（RBC）表现出良好的生物相容性，没有明显的不良反应。体内动物试验表明，通过及时有效的抗菌治疗，可以控制炎症反应，促进伤口愈合，局部毒性可忽略不计。这种具有生物相容性和抗菌性的 γ-PGA-TMPyP 纳米纤维垫在实际的抗感染应用中可能具有很大的潜力，特别是在伤口敷料应用中。
