南瓜籽提取物对原头蚴的体外作用
In Vitro Effects of Pumpkin () Seed Extracts on Protoscoleces.
作者信息
Hesari Zahra, Sharifdini Meysam, Sharifi-Yazdi Mohammad Kazem, Ghafari Saeedeh, Ghasemi Sara, Mahmoudi Shahram, Mohebali Mehdi, Nikmanesh Bahram
机构信息
Department of Pharmaceutics, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
出版信息
Iran J Parasitol. 2020 Jan-Mar;15(1):76-83.
BACKGROUND
parasite causes a zoonotic disease which is important for public and veterinary health. Since pumpkin seeds ( sp.) are used as traditional vermifuge in Iran, they may be a potential herbal anthelmintic.
METHODS
This study was designed in 2016 to evaluate the in vitro scolicidal effect of seeds form northern part of Iran. Hydroalcoholic and petroleum ether extracts were prepared by maceration and soxhlet respectively. Both extracts with four different concentrations (100, 10, 1, 0.1 mg/ml) were incubated against protoscoleces in 5, 15, 30 and 60 min.
RESULTS
Maximum mortality was 16% with 1% hydroalcoholic extract in 60 min. The highest mortality with organic extract was 4% with 10% concentration in 60 min (=0.015).
CONCLUSION
Since highest mortality was 16%, the extract did not reach to LD50 (50% mortality). Therefore, the potency of the total extract is not sufficient as potential scolicidal drug.
背景
寄生虫引发一种人畜共患病，对公共卫生和兽医健康都很重要。由于南瓜籽（南瓜属）在伊朗被用作传统驱虫剂，它们可能是一种潜在的草药驱虫药。
方法
本研究于2016年设计，旨在评估伊朗北部南瓜籽的体外杀头节效果。分别通过浸渍法和索氏提取法制备水醇提取物和石油醚提取物。将两种提取物的四种不同浓度（100、10、1、0.1毫克/毫升）与原头节一起孵育5、15、30和60分钟。
结果
60分钟时，1%水醇提取物的最高死亡率为16%。有机提取物在60分钟时10%浓度的最高死亡率为4%（P=0.015）。
结论
由于最高死亡率为16%，提取物未达到半数致死剂量（50%死亡率）。因此，总提取物作为潜在杀头节药物的效力不足。
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