Probiotics-Containing Yogurt Ingestion and Eradication Can Restore Fecal Dysbiosis in -Infected Children.

This study investigated the compositional differences in fecal microbiota between children with and without infection and tested whether probiotics-containing yogurt and bacterial eradication improve -related dysbiosis. Ten -infected children and 10 controls ingested probiotics-containing yogurt for 4 weeks. Ten-day triple therapy plus yogurt was given to the infected children on the 4th week. Fecal samples were collected at enrollment, after yogurt ingestion, and 4 weeks after successful eradication for cytokines and microbiota analysis using ELISA and metagenomic sequencing of the V4 region of the gene, respectively. The results showed -infected children had significantly higher levels of fecal TGF-β1 than those who were not infected. Eight of 295 significantly altered OTUs in the -infected children were identified. Among them, the abundance of was significantly lower in the -infected children, and then increased after yogurt ingestion and successful bacterial eradication. We further confirmed probiotics promoted growth in vitro and in ex vivo using real-time PCR. Moreover, supernatant significantly ameliorated lipopolysaccharide-induced IL-8 in HT-29 cells. In conclusions, Probiotics-containing yogurt ingestion and eradication can restore the decrease of fecal in -infected children.