Yang Yao-Jong, Chen Peng-Chieh, Lai Fu-Ping, Tsai Pei-Jane, Sheu Bor-Shyang
Departments of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70428, Taiwan.
Institute of Clinical Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70428, Taiwan.
Biomedicines. 2020 Jun 1;8(6):146. doi: 10.3390/biomedicines8060146.
This study investigated the compositional differences in fecal microbiota between children with and without infection and tested whether probiotics-containing yogurt and bacterial eradication improve -related dysbiosis. Ten -infected children and 10 controls ingested probiotics-containing yogurt for 4 weeks. Ten-day triple therapy plus yogurt was given to the infected children on the 4th week. Fecal samples were collected at enrollment, after yogurt ingestion, and 4 weeks after successful eradication for cytokines and microbiota analysis using ELISA and metagenomic sequencing of the V4 region of the gene, respectively. The results showed -infected children had significantly higher levels of fecal TGF-β1 than those who were not infected. Eight of 295 significantly altered OTUs in the -infected children were identified. Among them, the abundance of was significantly lower in the -infected children, and then increased after yogurt ingestion and successful bacterial eradication. We further confirmed probiotics promoted growth in vitro and in ex vivo using real-time PCR. Moreover, supernatant significantly ameliorated lipopolysaccharide-induced IL-8 in HT-29 cells. In conclusions, Probiotics-containing yogurt ingestion and eradication can restore the decrease of fecal in -infected children.
本研究调查了感染儿童与未感染儿童粪便微生物群的组成差异，并测试了含益生菌的酸奶和细菌根除是否能改善相关的生态失调。10名感染儿童和10名对照儿童摄入含益生菌的酸奶4周。在第4周，对感染儿童给予为期10天的三联疗法加酸奶。分别在入组时、摄入酸奶后以及成功根除后4周采集粪便样本，用于通过ELISA和对基因V4区域进行宏基因组测序分析细胞因子和微生物群。结果显示，感染儿童的粪便TGF-β1水平显著高于未感染儿童。在感染儿童中，295个显著改变的OTU中有8个被鉴定出来。其中，在感染儿童中，其丰度显著降低，在摄入酸奶和成功根除细菌后增加。我们使用实时PCR进一步证实益生菌在体外和体内促进了其生长。此外，其培养上清液显著改善了脂多糖诱导的HT-29细胞中IL-8的水平。总之，摄入含益生菌的酸奶和根除细菌可以恢复感染儿童粪便中该菌的减少。
