用于组织病理学的组织微波刺激固定与快速处理
MICROWAVE STIMULATED FIXATION AND RAPID PROCESSING OF TISSUE FOR HISTOPATHOLOGY.
作者信息
Sivadas P, Kumar Harsh, Lakshmanan C, Bhardwaj J R
机构信息
Director AFMS (Med Res), Office of DGAFMS, New Delhi 110011.
Reader, Dept of Pathology. A PMC, Pune.
出版信息
Med J Armed Forces India. 1996 Jul;52(3):157-160. doi: 10.1016/S0377-1237(17)30791-8. Epub 2017 Jun 26.
A rapid method for obtaining high quality paraffin sections is described. This method enables preparation of paraffin blocks in about 20 minutes and is based on microwave stimulated tissue fixation and processing. A total of 200 tissues were processed. Tissue blocks of size 1×1×0.5 were found ideal for processing. Three protocols were evaluated for tissue processing using different timings of exposure to graded alcohols, chloroform and wax. It was observed that utilizing an additional step of exposure to 70 per cent alcohol during tissue dehydration resulted in preparation of paraffin blocks which were light microscopically excellent and highly comparable to conventional methods.
本文描述了一种获得高质量石蜡切片的快速方法。该方法基于微波刺激组织固定和处理，能够在约20分钟内制备石蜡块。共处理了200个组织。发现尺寸为1×1×0.5的组织块最适合处理。评估了三种使用不同分级酒精、氯仿和石蜡暴露时间的组织处理方案。结果发现，在组织脱水过程中增加一步70%酒精暴露，可制备出光学显微镜下质量优异且与传统方法高度可比的石蜡块。
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