基于芯片和生物信息学分析的人前列腺癌中环状RNA的差异表达谱
Differential expression profiles of circRNAs in human prostate cancer based on chip and bioinformatic analysis.
作者信息
Ge Shengyang, Sun Chuanyu, Hu Qingfeng, Guo Yijun, Xia Guowei, Mi Yuanyuan, Zhu Lijie
机构信息
Department of Urology, Huashan Hospital, Fudan University Shanghai, P. R. China.
Department of Urology, Shanghai Jing'an District Central Hospital (Jing'an Branch of Huashan Hospital Affiliated to Fudan University) Shanghai, P. R. China.
出版信息
Int J Clin Exp Pathol. 2020 May 1;13(5):1045-1052. eCollection 2020.
BACKGROUND
Increasing evidence suggests that circRNAs are involved in the pathogenesis of multiple kinds of cancer. Nevertheless, the differential expression of circRNAs in prostate cancer (PCA) is rarely reported.
MATERIAL/METHOD: In our present analyses, circRNAs expression profiles were identified in PCA, based on 5 pairs of PCA and matched non-PCA tissues using circRNA chips.
RESULTS
A number of 749 differential circRNAs were expressed between PCA tumor and paracancerous tissues (Fold Change, FC ≥ 2.0 and < 0.05): 261 were upregulated, whereas 487 were downregulated in PCA tissues. Gene ontology and KEGG pathway analyses indicated that many of the circRNAs are related to carcinogenesis. Circ_0033074 and circ_0016064 both showed changes of maximum magnitude among differentially expressed circRNAs.
CONCLUSIONS
Our study detected a relative comprehensive differential map of circRNAs in PCA, which may become novel biomarkers for diagnosis, treatment and follow-up in the future.
背景
越来越多的证据表明，环状RNA（circRNAs）参与多种癌症的发病机制。然而，circRNAs在前列腺癌（PCA）中的差异表达鲜有报道。
材料/方法：在我们目前的分析中，基于5对PCA组织和匹配的非PCA组织，使用circRNA芯片鉴定了PCA中的circRNAs表达谱。
结果
PCA肿瘤组织与癌旁组织之间有749种差异circRNAs表达（折叠变化，FC≥2.0且<0.05）：PCA组织中261种上调，487种下调。基因本体论和KEGG通路分析表明，许多circRNAs与致癌作用有关。Circ_0033074和circ_0016064在差异表达的circRNAs中均表现出最大幅度的变化。
结论
我们的研究检测到PCA中相对全面的circRNAs差异图谱，这可能在未来成为诊断、治疗和随访的新型生物标志物。
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