Clinic for Orthopedics and Trauma Surgery, University Hospital Bonn, 53127 Bonn, Germany.
Clinic for Cardiothoracic Surgery, University Hospital Cologne, 50937 Cologne, Germany.
Int J Mol Sci. 2020 Jun 19;21(12):4382. doi: 10.3390/ijms21124382.
Mesenchymal stromal cells (MSC) harvested in different tissues from the same donor exhibit different phenotypes. Each phenotype is not only characterized by a certain pattern of cell surface markers, but also different cellular functionalities. Only recently were different harvesting and processing techniques found to contribute to this phenomenon as well. This study was therefore set up to investigate proteomic and functional properties of human bone marrow-derived MSCs (hBM-MSC). These were taken from the same tissue and donor site but harvested either as aspirate or bone chip cultures. Both MSC populations were profiled for MSC markers defined by the International Society for Cellular Therapy (ISCT), MSC markers currently under discussion and markers of particular interest. While classic ISCT MSC markers did not show any significant difference between aspirate and outgrowth hBM-MSCs, our additional characterization panel revealed distinct patterns of differentially expressed markers. Furthermore, hBM-MSCs from aspirate cultures demonstrated a significantly higher osteogenic differentiation potential than outgrowth MSCs, which could be confirmed using a transcriptional approach. Our comparison of MSC phenotypes obtained by different harvesting techniques suggests the need of future standardized harvesting, processing and phenotyping procedures in order to gain better comparability in the MSC field.
间充质基质细胞（MSC）从同一供体的不同组织中采集，表现出不同的表型。每种表型不仅具有特定的细胞表面标志物模式，而且具有不同的细胞功能。直到最近，人们才发现不同的采集和处理技术也促成了这种现象。因此，本研究旨在研究人骨髓来源的间充质基质细胞（hBM-MSC）的蛋白质组学和功能特性。这些细胞均取自同一组织和供体部位，但分别作为抽吸物或骨片培养物采集。两种 MSC 群体均进行了国际细胞治疗学会（ISCT）定义的 MSC 标志物、目前正在讨论的 MSC 标志物和特别感兴趣的标志物的特征分析。虽然经典的 ISCT MSC 标志物在抽吸物和体外生长 hBM-MSC 之间没有显示出任何显著差异，但我们的附加特征分析面板揭示了差异表达标志物的不同模式。此外，抽吸培养物中的 hBM-MSCs 表现出比体外生长 MSC 更高的成骨分化潜力，这可以通过转录方法得到证实。我们对不同采集技术获得的 MSC 表型进行比较，表明需要未来标准化的采集、处理和表型分析程序，以便在 MSC 领域获得更好的可比性。
