Department of Medicinal Plant Resources, Andong National University, Andong, Geongsangbuk 36729, Republic of Korea.
Department of Medicinal Plant Science, Jungwon University, Geosan, Chungcheongbuk 28024, Republic of Korea.
Mol Med Rep. 2020 Sep;22(3):2076-2084. doi: 10.3892/mmr.2020.11258. Epub 2020 Jun 18.
Abeliophyllum distichum Nakai is a Korean endemic plant of the Oleaceae family that contains acteoside, a glycosylated caffeic acid, with neuroprotective, anti‑inflammatory and antibacterial properties. Previous studies, involving Accelerated Chromatographic Isolation, a high‑performance liquid chromatography‑photodiode array detector and a liquid chromatograph‑mass selective detector, isolated and identified acteoside in A. distichum (AAD) and documented its antioxidant and anti‑inflammatory activities. The aim of the present study was to determine whether AAD could protect from DNA damage by reducing oxidative stress. AAD treatment protected plasmid DNA against damage to DNA double‑strands induced by reactive oxygen species (ROS) and decreased the levels of phosphorylated p53 and γ‑H2AX in ROS‑treated NIH 3T3 cells. These findings suggested that AAD could reduce ROS‑mediated cellular damage and may represent an effective, natural antioxidant with the ability to protect genetic material.
朝鲜特有植物山茱萸科垂枝连翘含有具有神经保护、抗炎和抗菌特性的环烯醚萜苷类化合物——獐牙菜苦苷。先前的研究涉及加速色谱分离、高效液相色谱-光电二极管阵列检测器和液相色谱-质量选择检测器，从垂枝连翘中分离并鉴定了獐牙菜苦苷（AAD），并记录了其抗氧化和抗炎活性。本研究旨在确定 AAD 是否可以通过降低氧化应激来保护 DNA 免受损伤。AAD 处理可防止质粒 DNA 受到活性氧（ROS）诱导的 DNA 双链损伤，并降低 ROS 处理的 NIH 3T3 细胞中磷酸化 p53 和 γ-H2AX 的水平。这些发现表明，AAD 可以减少 ROS 介导的细胞损伤，并且可能是一种有效的天然抗氧化剂，能够保护遗传物质。
