Kolan Krishna C R, Semon Julie A, Bromet Bradley, Day Delbert E, Leu Ming C
Department of Mechanical and Aerospace Engineering, Missouri University of Science and Technology, Rolla, Missouri, USA.
Department of Biological Sciences, Missouri University of Science and Technology, Rolla, Missouri, USA.
Int J Bioprint. 2019 Jul 12;5(2.2):204. doi: 10.18063/ijb.v5i2.2.204. eCollection 2019.
Three-dimensional (3D) bioprinting technologies have shown great potential in the fabrication of 3D models for different human tissues. Stem cells are an attractive cell source in tissue engineering as they can be directed by material and environmental cues to differentiate into multiple cell types for tissue repair and regeneration. In this study, we investigate the viability of human adipose-derived mesenchymal stem cells (ASCs) in alginate-gelatin (Alg-Gel) hydrogel bioprinted with or without bioactive glass. Highly angiogenic borate bioactive glass (13-93B3) in 50 wt% is added to polycaprolactone (PCL) to fabricate scaffolds using a solvent-based extrusion 3D bioprinting technique. The fabricated scaffolds with 12 × 12 × 1 mm in overall dimensions are physically characterized, and the glass dissolution from PCL/glass composite over a period of 28 days is studied. Alg-Gel composite hydrogel is used as a bioink to suspend ASCs, and scaffolds are then bioprinted in different configurations: Bioink only, PCL+bioink, and PCL/glass+bioink, to investigate ASC viability. The results indicate the feasibility of the solvent-based bioprinting process to fabricate 3D cellularized scaffolds with more than 80% viability on day 0. The decrease in viability after 7 days due to glass concentration and static culture conditions is discussed. The feasibility of modifying Alg-Gel with 13-93B3 glass for bioprinting is also investigated, and the results are discussed.
三维（3D）生物打印技术在制造不同人体组织的3D模型方面已显示出巨大潜力。干细胞是组织工程中一种有吸引力的细胞来源，因为它们可以在材料和环境线索的引导下分化为多种细胞类型，用于组织修复和再生。在本研究中，我们研究了人脂肪来源间充质干细胞（ASC）在添加或不添加生物活性玻璃的藻酸盐-明胶（Alg-Gel）水凝胶生物打印中的活力。将50 wt%的高血管生成性硼酸盐生物活性玻璃（13-93B3）添加到聚己内酯（PCL）中，使用基于溶剂的挤出3D生物打印技术制造支架。对整体尺寸为12×12×1 mm的制造支架进行物理表征，并研究PCL/玻璃复合材料在28天内的玻璃溶解情况。Alg-Gel复合水凝胶用作生物墨水来悬浮ASC，然后将支架以不同配置进行生物打印：仅生物墨水、PCL+生物墨水和PCL/玻璃+生物墨水，以研究ASC的活力。结果表明基于溶剂的生物打印过程在第0天制造具有超过80%活力的3D细胞化支架的可行性。讨论了由于玻璃浓度和静态培养条件导致的7天后活力下降情况。还研究了用13-93B3玻璃修饰Alg-Gel用于生物打印的可行性，并对结果进行了讨论。
