Department of Cardiothoracic Surgery, Nanjing Drum Tower Hospital, Nanjing Medical University, Nanjing, China.
School of Life Science, Nanjing University, Nanjing, China.
Aging (Albany NY). 2020 Jun 28;12(14):15002-15010. doi: 10.18632/aging.103559.
Changes in expression of long non-coding RNAs (lncRNAs) in plasma exosomes can be useful for diagnosis of cancer patients. Here, we conducted a four-stage study to identify plasma exosome lncRNAs with diagnostic potential in esophageal squamous cell carcinoma (ESCC). First, plasma exosome lncRNA expression profiles were examined in ESCC patients, esophagitis patients, and healthy controls using RNA sequencing. Differentially expressed plasma exosome lncRNAs from the lncRNA expression profile were then evaluated by qRT-PCR in a large cohort of ESCC patients, esophagitis patients, and healthy controls. Expression levels of the lncRNAs NR_039819, NR_036133, NR_003353, ENST00000442416.1, and ENST00000416100.1 were significantly higher in exosomes from ESCC patients than non-cancer controls. We also confirmed that levels of these five plasma exosome lncRNAs decreased markedly in ESCC patients after surgery. Our results suggest that these five exosome lncRNAs may serve as highly effective, noninvasive biomarkers for ESCC diagnosis.
血浆外泌体长非编码 RNA（lncRNA）表达的变化可用于癌症患者的诊断。在此，我们进行了一项四阶段研究，以鉴定具有诊断潜力的食管鳞状细胞癌（ESCC）患者血浆外泌体 lncRNA。首先，我们使用 RNA 测序检查了 ESCC 患者、食管炎患者和健康对照者的血浆外泌体 lncRNA 表达谱。然后，通过 qRT-PCR 在大量 ESCC 患者、食管炎患者和健康对照者中评估了 lncRNA 表达谱中差异表达的血浆外泌体 lncRNA。来自 ESCC 患者的外泌体中 NR_039819、NR_036133、NR_003353、ENST00000442416.1 和 ENST00000416100.1 的 lncRNA 表达水平明显高于非癌症对照者。我们还证实，这些五种血浆外泌体 lncRNA 在 ESCC 患者手术后明显降低。我们的结果表明，这五种外泌体 lncRNA 可能是 ESCC 诊断的高效、非侵入性生物标志物。
