"Ilie Murgulescu" Institute of Physical Chemistry, Romanian Academy, 202 Spl. Independentei, 060021 Bucharest, Romania.
National Institute of Research and Development for Biological Sciences, 296 Spl. Independentei, 060031 Bucharest, Romania.
Int J Biol Macromol. 2022 Jun 30;211:410-424. doi: 10.1016/j.ijbiomac.2022.05.070. Epub 2022 May 13.
A facile, green synthesis methodology to obtain zinc oxide nanoparticles using three polysaccharide gums (Acacia gum, Guar gum and Xanthan gum) of biological origin was developed. Subsequently, biosynthesized zinc oxide nanoparticles were incorporated into a sustainable chitosan hydrogel matrix functionalized with propolis extract. This study has revealed that the selected polysaccharides as chelates represents a suitable approach to synthesize ZnO nanoparticles of particular interest with controlled morphology. The formation of ZnO nanoparticles using polysaccharide gums was confirmed by FTIR, XRD, UV-Vis spectroscopy, thermal analysis, SEM, Raman and photoluminescence spectroscopies. The rheological behaviour of obtained hydrogels was evaluated. The AFM studies demonstrate that all synthesized chitosan incorporated ZnO composites hydrogels functionalized with propolis extract exhibit corrugated topographies. The present study highlights the possible incorporation of various guest molecules into hydrogel matrix due to its tuneable morphologies. The obtained hydrogel composites were cytocompatible in L929 fibroblast cell culture, in a range of concentrations between 50 and 1000 μg/mL, as assessed by MTT, LDH and Live/Dead double staining assays. By enhancing the biological properties, these novel green hydrogels show attractive superior performance in a wide concentration range to develop future in vivo suitable natural platforms as effective delivery systems of pharmacologic agents for biomedical applications.
采用三种生物来源的多糖胶（阿拉伯胶、瓜尔胶和黄原胶），开发了一种简便、绿色的合成氧化锌纳米粒子的方法。随后，将生物合成的氧化锌纳米粒子掺入具有蜂胶提取物的可持续性壳聚糖水凝胶基质中。这项研究表明，所选多糖作为螯合物，代表了一种合适的方法，可以合成具有特定形态的、特别感兴趣的 ZnO 纳米粒子。多糖胶合成 ZnO 纳米粒子的形成通过傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）、紫外-可见光谱、热分析、扫描电子显微镜（SEM）、拉曼和光致发光光谱进行了确认。还评估了获得的水凝胶的流变行为。原子力显微镜（AFM）研究表明，所有合成的壳聚糖掺入的 ZnO 复合材料水凝胶都具有蜂胶提取物的功能，表现出波纹状形貌。本研究强调了由于其可调形态，各种客体分子可能被掺入水凝胶基质中。在所研究的浓度范围内（50 至 1000μg/mL），通过 MTT、LDH 和 Live/Dead 双重染色测定，评价了所得水凝胶复合材料在 L929 成纤维细胞培养中的细胞相容性。通过增强这些新型绿色水凝胶的生物特性，在广泛的浓度范围内展示出有吸引力的优越性能，为开发未来适合体内应用的天然药物输送系统提供了有效的药物载体。
