Roche Y, Fay M, Gougerot-Pocidalo M A
Laboratoire de Biologie-Odontologie, Université Paris, France.
J Antimicrob Chemother. 1988 May;21(5):597-607. doi: 10.1093/jac/21.5.597.
The effects of penicillin, macrolides (spiramycin and erythromycin), cephalosporins (cefaclor and cefadroxil), tetracycline (doxycycline) and quinolones (pefloxacin, ciprofloxacin and ofloxacin) on extracellular and cell-associated interleukin 1 (IL-1) activity from human adherent mononuclear leucocyte cells were investigated in vitro. When cells were treated with an antibiotic concentration of 10 mg/l, no apparent effect could be detected for penicillin, erythromycin, cephalosporins or quinolones, while a slight increase of extracellular IL-1 activity associated with a decrease of intracellular IL-1 activity was observed with spiramycin and doxycycline. When high antibiotic concentration were used, extracellular IL-1 activity was increased by macrolides and tetracycline, while both cell-associated and class II human monocyte antigen expression were decreased. A toxic effect may have been exerted by these antimicrobial agents, since cell viability was altered when they were used at high concentrations. In contrast, extracellular IL-1 activity was found to be decreased by quinolones and cephalosporins. Intracellular IL-1 activity was also decreased by cephalosporins, while quinolones did not modify either cell-associated IL-1 activity or class II human monocyte antigen expression. The effect induced by quinolones and cephalosporins occurred without modification of cell viability. IL-1 activity was shown to be affected by antibiotics over the same range of concentrations which are known to inhibit mononuclear leucocyte proliferation. Our data may help in defining the mechanism by which the mitogen-induced mononuclear proliferative response is suppressed by antimicrobial agents since this appears to involve the inhibition of IL-1 production or of its release.
体外研究了青霉素、大环内酯类抗生素（螺旋霉素和红霉素）、头孢菌素类抗生素（头孢克洛和头孢羟氨苄）、四环素（强力霉素）和喹诺酮类抗生素（培氟沙星、环丙沙星和氧氟沙星）对人黏附单核白细胞细胞外及细胞相关白细胞介素1（IL-1）活性的影响。当细胞用10mg/L的抗生素浓度处理时，未检测到青霉素、红霉素、头孢菌素类抗生素或喹诺酮类抗生素有明显作用，而螺旋霉素和强力霉素使细胞外IL-1活性略有增加，同时细胞内IL-1活性降低。当使用高浓度抗生素时，大环内酯类抗生素和四环素使细胞外IL-1活性增加，而细胞相关的II类人单核细胞抗原表达均降低。这些抗菌药物可能产生了毒性作用，因为高浓度使用时细胞活力发生了改变。相比之下，喹诺酮类抗生素和头孢菌素类抗生素使细胞外IL-1活性降低。头孢菌素类抗生素也使细胞内IL-1活性降低，而喹诺酮类抗生素对细胞相关IL-1活性或II类人单核细胞抗原表达均无影响。喹诺酮类抗生素和头孢菌素类抗生素诱导的作用未改变细胞活力。IL-1活性在已知抑制单核白细胞增殖的相同浓度范围内受到抗生素的影响。我们的数据可能有助于确定抗菌药物抑制丝裂原诱导的单核细胞增殖反应的机制，因为这似乎涉及抑制IL-1的产生或释放。
