Universidade Federal Fluminense - UFF, School of Dentistry, Department of Pediatric Dentistry, Niterói, RJ, Brazil.
Braz Oral Res. 2020 Jun 24;34:e061. doi: 10.1590/1807-3107bor-2020.vol34.0061.
The purpose of this double-blind, randomized, crossover in situ study is to compare remineralization of preformed enamel lesions by casein phosphopeptide-stabilized amorphous calcium phosphate (CPP-ACP) and fluoride dentifrice products. During each of four 10-day experimental legs, 10 participants wore intraoral removable palatal acrylic appliances with four human enamel slabs with preformed lesions. A 0.03-mL treatment paste was dripped extraorally onto the enamel blocks once a day for 3 min. The four randomly allocated treatments were as follows: CO- Control: silica dentifrice without fluoride; MP: MI Paste; MPP: MI Paste Plus and FD: Fluoride dentifrice - 1100 ppm F as NaF). Knoop surface hardness (SH) test was performed in three stages (T0 - sound enamel, T1 - after preformed lesion, and T2 - after treatment) and the cross-sectional hardness (CSH) test was performed after treatment using a 50-gram Knoop load for 15 s. Knoop hardness number (KHN) was similar between treatments. %SHr was significantly higher in the MP, FD, and MPP when compared to CO group (Kruskal-Wallis and Mann-Whitney tests, p < 0.05). Harder enamel was found in MP (75 μm) and FD groups at 75 to 175 μm. Treatment with DF, MP, and MPP promoted an increase of 20.27%, 19.24%, and 14.71%, respectively, in Integral Hardness Change (ΔIHC) when compared to CO (p<0.05). Remineralizing agents (MP, MPP, and DF) were able to inhibit demineralization of human enamel subjected to high cariogenic challenge in situ. DF had the greatest preventive potential against the progression of carious lesions.
本双盲、随机、交叉原位研究的目的是比较酪蛋白磷酸肽稳定的无定形磷酸钙（CPP-ACP）和含氟牙膏产品对已形成釉质龋的再矿化作用。在四个为期 10 天的实验阶段中，每个阶段有 10 名参与者佩戴口腔内可移动的腭部丙烯酸器具，每个器具上有四个带有已形成的龋损的人釉质板块。每天一次将 0.03 毫升处理糊剂滴到釉质块上，持续 3 分钟。四种随机分配的处理方法如下：CO-对照：不含氟的二氧化硅牙膏；MP：MI 糊剂；MPP：MI 糊剂 Plus 和 FD：含氟牙膏-1100ppmF 以 NaF 计）。使用 50 克的克努普硬度计在三个阶段（T0-完好釉质、T1-形成龋损后、T2-处理后）进行表面硬度（SH）测试，并在处理后使用 50 克的克努普硬度计在 15 秒内进行横截面试验（CSH）。Knoop 硬度值（KHN）在处理方法之间相似。与 CO 组相比，MP、FD 和 MPP 组的 %SHr 显著更高（Kruskal-Wallis 和 Mann-Whitney 检验，p<0.05）。在 75 至 175μm 范围内，MP（75μm）和 FD 组的釉质硬度更高。与 CO 组相比，DF、MP 和 MPP 处理后，整体硬度变化（ΔIHC）分别增加了 20.27%、19.24%和 14.71%（p<0.05）。再矿化剂（MP、MPP 和 DF）能够抑制在高致龋挑战原位下人釉质的脱矿。DF 对龋病进展具有最大的预防潜力。
