快速诊断检测应对全球麻疹监测挑战。
Rapid diagnostic tests to address challenges for global measles surveillance.
机构信息
Virus Reference Department, National Infection Service, Public Health England, London, UK; Laboratório de Vírus Respiratórios e do Sarampo, Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, Rio de Janeiro, Brazil.
Virus Reference Department, National Infection Service, Public Health England, London, UK.
出版信息
Curr Opin Virol. 2020 Apr;41:77-84. doi: 10.1016/j.coviro.2020.05.007. Epub 2020 Jun 29.
Recently, a lateral flow rapid diagnostic test (RDT) with good accuracy has been described. This test enables measles specific IgM antibody detection in serum, capillary blood and oral fluid. RDTs have the potential to transform measles surveillance by allowing real-time case confirmation outside of central/regional laboratories and by facilitating a timely public health response. Measles virus genes can also be amplified and sequenced consistently from dried IgM-positive RDTs stored outside of cold chain, which will enable more complete virologic surveillance. Critical questions remain regarding operational use of RDTs as part of global measles surveillance. Projects to evaluate RDT use as part of national surveillance programs and to commercialize the RDT are underway.
最近，一种具有良好准确性的侧向流动快速诊断检测（RDT）已经被描述。这种检测方法可以在血清、毛细血管血和口腔液中检测麻疹特异性 IgM 抗体。RDT 有可能通过允许在中央/区域实验室之外实时确认病例，并通过促进及时的公共卫生应对，来改变麻疹监测。从冷藏链外储存的干燥 IgM 阳性 RDT 中，也可以一致地扩增和测序麻疹病毒基因，这将使病毒学监测更加完整。关于将 RDT 作为全球麻疹监测的一部分进行操作使用，仍存在一些关键问题。正在开展评估 RDT 在国家监测计划中使用情况并将 RDT 商业化的项目。
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