Harkiss G D, Hendrie F M, Thompson D
University of Edinburgh, Department of Veterinary Pathology, UK.
J Clin Immunol. 1988 Jul;8(4):319-31. doi: 10.1007/BF00916560.
Patients with systemic lupus erythematosus were examined for the presence of serum antibodies reactive with phospholipids (PL) extracted from the membranes of Klebsiella K30 (K30PL). Affinity-purified anti-K30PL antibodies were tested for their ability to cross-react with other PL antigens or DNA and for the presence of idiotypic markers known to be associated with anti-DNA antibodies or antibodies to Klebsiella K30 polysaccharide (K30p). Affinity-purified antibodies to K30PL, phosphatidylserine (PS), or phosphatidylinositol (PL) uniformly cross-reacted with each other. Analysis of the PL preparations by thin-layer chromatography and reversed-phase high-performance liquid chromatography (HPLC) showed the presence of several components. Lupus sera reacted with one component mainly in the HPLC-fractionated K30PL preparation, although this component appeared to be present in the other PL preparations. Direct-binding and inhibition studies showed that affinity-purified antibodies to the K30PL extract, PS, or PI reacted poorly with DNA. However, the anti-K30PL antibodies possessed a prominent anti-DNA idiotypic marker (AM Id) in 61% of the patients and an anti-K30p idiotypic marker (SP Id) in 94% of the patients. The results thus show that anti-K30PL antibodies are idiotypically related to anti-DNA and anti-K30p antibody subpopulations, although they do not share the same antigen-binding characteristics.
对系统性红斑狼疮患者进行检测,以确定其血清中是否存在与从肺炎克雷伯菌K30(K30PL)膜中提取的磷脂(PL)发生反应的抗体。对亲和纯化的抗K30PL抗体进行检测,以确定其与其他PL抗原或DNA发生交叉反应的能力,以及是否存在已知与抗DNA抗体或肺炎克雷伯菌K30多糖(K30p)抗体相关的独特型标记。亲和纯化的抗K30PL、磷脂酰丝氨酸(PS)或磷脂酰肌醇(PI)抗体彼此均能发生交叉反应。通过薄层色谱和反相高效液相色谱(HPLC)对PL制剂进行分析,结果显示存在几种成分。狼疮血清主要与HPLC分离的K30PL制剂中的一种成分发生反应,尽管该成分似乎也存在于其他PL制剂中。直接结合和抑制研究表明,亲和纯化的抗K3PL提取物、PS或PI抗体与DNA的反应较弱。然而,在61%的患者中,抗K30PL抗体具有显著的抗DNA独特型标记(AM Id),在94%的患者中具有抗K30p独特型标记(SP Id)。因此,结果表明,抗K30PL抗体在独特型上与抗DNA和抗K30p抗体亚群相关,尽管它们不具有相同的抗原结合特性。