Lin Min, Xiong Hui, Xiang Xuechuan, Zhou Zelin, Liang Lifeng, Mei Zhinan
School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.
Institute of Ethnomedicine, South-Central University for Nationalities, Wuhan, China.
Front Microbiol. 2020 Jun 18;11:1257. doi: 10.3389/fmicb.2020.01257. eCollection 2020.
(L.) R. Br. is an important perennial terrestrial photosynthetic orchid species whose microbiomes are considered to play an important role in helping its germination and growth. However, the assemblage of root-associated microbial communities is poorly understood. The compositions of fungal and bacterial communities from the roots and corresponding soil samples in across distinct biogeographical regions from two significantly different altitudes were characterized at the vegetative and reproductive growth stages. The geographical location, developmental stage and compartment were factors contributing to microbiome variation in . Predominant fungal taxa include , , and , whereas , , , , , , and were predominant bacterial taxa. Using as a model, the structural and functional composition in root-associated microbiomes were comprehensive analyzed. Contrary to previous studies, biogeography was the main factor influencing the microbial community in this study. Besides, compartment and developmental stage should also be considered to analyze the variation of microbiota composition. Although the microbial composition varied greatly by location, the symbiotic microorganisms of still have certain specificity. This study gives an abundant information of root-associated microbiomes and provides new clues to better understanding the factors affecting the composition and diversity of fungal/bacterial communities associated with orchids. Our results also laying a foundation for harnessing the microbiome for sustainable cultivation. Moreover, these results might be generally applicable to other orchidaceae plants.
（L.）R. Br. 是一种重要的多年生陆生光合兰花物种，其微生物群被认为在帮助其萌发和生长方面发挥着重要作用。然而，人们对其根际微生物群落的组合了解甚少。在营养生长和生殖生长阶段，对来自两个显著不同海拔的不同生物地理区域的根和相应土壤样本中的真菌和细菌群落组成进行了表征。地理位置、发育阶段和区室是导致（该兰花物种）微生物群变化的因素。主要的真菌类群包括（列举的真菌类群名称），而（列举的细菌类群名称）是主要的细菌类群。以（该兰花物种）为模型，对其根际微生物群的结构和功能组成进行了全面分析。与先前的研究相反，生物地理学是本研究中影响微生物群落的主要因素。此外，在分析微生物群组成的变化时，还应考虑区室和发育阶段。尽管微生物组成因地点而异，但（该兰花物种）的共生微生物仍具有一定的特异性。本研究提供了关于（该兰花物种）根际微生物群的丰富信息，并为更好地理解影响兰花相关真菌/细菌群落组成和多样性的因素提供了新线索。我们的结果也为利用微生物群实现（该兰花物种）的可持续种植奠定了基础。此外，这些结果可能普遍适用于其他兰科植物。
