College of Animal Science and Technology, Hunan Agricultural University, Hunan Co-Innovation of Animal Production Safety, Changsha, Hunan, China.
CAS Key Laboratory of Agro-ecological Processes in Subtropical Region, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, China.
J Appl Microbiol. 2021 Jan;130(1):217-232. doi: 10.1111/jam.14767. Epub 2020 Jul 20.
The study was conducted to investigate the effects of dietary Bacillus subtilis (BS) DSM 32315 on the intestinal microbiota composition and metabolites of weaned pigs.
Sixty-four piglets were allocated to two groups (control and BS), each group including eight replicates with four piglets. Dietary BS DSM 32315 increased (P < 0·05) the abundances of jejunal Leucobacter and Cupriavidus, ileal Thermus, Coprococcus and Bifidobacterium, as well as colonic Succiniclasticum; and increased the concentrations of ileal straight-chain fatty acids, colonic propionate, branched-chain fatty acids (BCFAs), and tyramine, but decreased (P < .05) the colonic indole concentration. The ileal and colonic microbial community structure tended to cluster into two groups. LEfSe analysis identified five microbial biomarkers in jejunum and eight biomarkers in ileum in the BS group, and three biomarkers in colon in the control group. The ileal Bifidobacterium abundance was positively correlated (P < 0·05) with isovalerate concentration, while the colonic Actinobacteria and Lactobacillus abundances were negatively correlated (P < 0·05) with indole concentration.
These findings suggest that dietary supplementation with BS DSM 32315 could alter the diversity, composition, and metabolites of intestinal microbiota in weaned piglets.
Weaned piglets are often accompanied with impaired gastrointestinal tract and intestinal disorder affecting their growth. This study demonstrated that dietary BS DSM 32315 presented a beneficial role in gut health via regulating intestinal microbiota composition and metabolites.
本研究旨在探讨枯草芽孢杆菌（BS）DSM 32315 对断奶仔猪肠道微生物群落组成和代谢物的影响。
将 64 头仔猪分为两组（对照组和 BS 组），每组包括 8 个重复，每个重复 4 头仔猪。日粮中添加 BS DSM 32315 增加了（P<0·05）空肠中 Leucobacter 和 Cupriavidus、回肠中 Thermus、Coprococcus 和双歧杆菌以及结肠中 Succiniclasticum 的丰度；并增加了回肠中直链脂肪酸、结肠中丙酸、支链脂肪酸（BCFAs）和酪胺的浓度，但降低了（P<0·05）结肠中吲哚的浓度。回肠和结肠微生物群落结构趋于聚类成两组。LEfSe 分析鉴定出 BS 组空肠中有 5 种微生物生物标志物，回肠中有 8 种生物标志物，对照组结肠中有 3 种生物标志物。回肠双歧杆菌丰度与异戊酸浓度呈正相关（P<0·05），而结肠放线菌和乳杆菌丰度与吲哚浓度呈负相关（P<0·05）。
这些发现表明，日粮中添加 BS DSM 32315 可以改变断奶仔猪肠道微生物群落的多样性、组成和代谢物。
断奶仔猪常伴有胃肠道受损和肠道紊乱，影响其生长。本研究表明，日粮 BS DSM 32315 通过调节肠道微生物群落组成和代谢物，对肠道健康具有有益作用。
