Kim Gil Soo, Kim Geum Jin, Lee Byeongsan, Oh Tae Hoon, Kwon Mincheol, Lee Jung-Sook, Jang Jun-Pil, Choi Hyukjae, Ko Sung-Kyun, Hong Young-Soo, Jang Jae-Hyuk, Ahn Jong Seog
Anticancer Agent Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, 28116, Korea.
Department of Biomolecular Science, KRIBB School of Bioscience, Korea University of Science and Technology, Daejeon, 34113, Korea.
J Antibiot (Tokyo). 2020 Dec;73(12):859-862. doi: 10.1038/s41429-020-0348-8. Epub 2020 Jul 10.
LC/MS-based chemical screening of culture extract led to a new highly oxygenated angucycline derivative, grecocycline D (1), from Streptomyces sp. KCB15JA014, isolated from a soil sample of Oedolgae in Jeju Island, Korea. The planar structure was determined on the basis of spectroscopic analysis, including 1D and 2D NMR techniques as well as HRESIMS and comparison with data from the literature. A relative and absolute configuration of 1 was assigned by ROESY experiment and electronic circular dichroism calculation. Compound 1 showed weak inhibitory activity against indoleamine 2,3-dioxygenase.
基于液相色谱-质谱联用的培养提取物化学筛选，从韩国济州岛牛岛土壤样品中分离得到的链霉菌属菌株KCB15JA014中发现了一种新的高度氧化的安古环素衍生物——格氏环素D（1）。通过光谱分析（包括一维和二维核磁共振技术以及高分辨电喷雾电离质谱）并与文献数据进行比较，确定了其平面结构。通过旋转坐标系核Overhauser效应光谱实验和电子圆二色性计算确定了化合物1的相对和绝对构型。化合物1对吲哚胺2,3-双加氧酶显示出微弱的抑制活性。
