Unit for Laboratory Animal Medicine, University of Michigan, Ann Arbor, MI, USA.
Biointerfaces Institute, University of Michigan, Ann Arbor, MI, USA.
Sci Rep. 2020 Jul 9;10(1):11401. doi: 10.1038/s41598-020-68395-3.
Urodynamic studies, used to understand bladder function, diagnose bladder disease, and develop treatments for dysfunctions, are ideally performed with awake subjects. However, in small and medium-sized animal models, anesthesia is often required for these procedures and can be a research confounder. This study compared the effects of select survival agents (dexmedetomidine, alfaxalone, and propofol) on urodynamic (Δpressure, bladder capacity, bladder compliance, non-voiding contractions, bladder pressure slopes) and anesthetic (change in heart rate [∆HR], average heart rate [HR], reflexes, induction/recovery times) parameters in repeated cystometrograms across five adult male cats. The urodynamic parameters under isoflurane and α-chloralose were also examined in terminal procedures for four cats. Δpressure was greatest with propofol, bladder capacity was highest with α-chloralose, non-voiding contractions were greatest with α-chloralose. Propofol and dexmedetomidine had the highest bladder pressure slopes during the initial and final portions of the cystometrograms respectively. Cats progressed to a deeper plane of anesthesia (lower HR, smaller ΔHR, decreased reflexes) under dexmedetomidine, compared to propofol and alfaxalone. Time to induction was shortest with propofol, and time to recovery was shortest with dexmedetomidine. These agent-specific differences in urodynamic and anesthetic parameters in cats will facilitate appropriate study-specific anesthetic choices.
尿动力学研究用于了解膀胱功能、诊断膀胱疾病和开发功能障碍治疗方法，理想情况下是在清醒的受试者中进行。然而，在中小动物模型中，这些程序通常需要麻醉，这可能会成为研究的混杂因素。本研究比较了几种存活剂（右美托咪定、阿法沙龙和丙泊酚）对五只成年雄性猫反复膀胱测压时尿动力学（Δ压力、膀胱容量、膀胱顺应性、非逼尿肌收缩、膀胱压力斜率）和麻醉（心率变化[ΔHR]、平均心率[HR]、反射、诱导/恢复时间）参数的影响。还在四只猫的终末程序中检查了异氟烷和α-氯醛下的尿动力学参数。在丙泊酚下，Δ压力最大，在α-氯醛下，膀胱容量最大，在α-氯醛下，非逼尿肌收缩最大。在膀胱测压图的初始和最后部分，丙泊酚和右美托咪定的膀胱压力斜率最高。与丙泊酚和阿法沙龙相比，猫在右美托咪定下进展到更深的麻醉平面（更低的 HR、更小的 ΔHR、反射减少）。丙泊酚诱导时间最短，右美托咪定恢复时间最短。这些猫在尿动力学和麻醉参数方面的特定药物差异将有助于进行适当的特定于研究的麻醉选择。
