Shamloo Amir, Yazdani Alireza, Saghafifar Fatemeh
Department of Mechanical Engineering Sharif University of Technology Tehran Iran.
Eng Life Sci. 2020 Apr 27;20(7):296-304. doi: 10.1002/elsc.202000001. eCollection 2020 Jul.
Identifying tumor cells from a pool of other cells has always been an appealing topic for different purposes. The objective of this study is to discriminate circulating tumor cells (CTCs) from blood cells for diagnostic purposes in a novel microfluidic device using two active methods: magnetophoresis and dielectrophoresis. The most specific feature of this device is the differentiation of CTCs without labeling them in order to achieve a more reliable and less complicated method. This device was analyzed and evaluated using finite element method. Four cell lines are separated in this device containing red blood cells, platelets, white blood cells, and CTCs. Primarily, red blood cells and platelets, which constitute the largest part of a blood sample, are removed in the magnetophoresis section. Remaining cells enter the dielectrophoresis part and based on their inherent dielectric properties and diameters, final separation occurs. In each step, different parameters are examined to obtain the maximum purification. The results demonstrate the potential of different CTCs separation by changing the effective parameters in the designed device based on the inherent properties of the cells.
从其他细胞群体中识别肿瘤细胞一直是出于不同目的而备受关注的话题。本研究的目的是在一种新型微流控装置中，使用磁泳和介电泳这两种主动方法，为诊断目的从血细胞中区分出循环肿瘤细胞（CTC）。该装置最独特的特点是在不标记CTC的情况下对其进行区分，以实现一种更可靠、更简单的方法。使用有限元方法对该装置进行了分析和评估。在该装置中分离了四种细胞系，包括红细胞、血小板、白细胞和CTC。首先，在磁泳部分去除构成血样最大部分的红细胞和血小板。剩余细胞进入介电泳部分，根据它们固有的介电特性和直径进行最终分离。在每个步骤中，都会检查不同参数以实现最大程度的纯化。结果表明，基于细胞的固有特性，通过改变设计装置中的有效参数，不同的CTC分离具有潜力。
