Zhang Yu, Gao Zhan, Wang Shaoli, Liu Jing, Paul Ness, He Tao, Liu Cunxu, Zhang Hongbin, Lv Yunlai, Cao Ru'an, Mao Wei, Wan Jianhua, Ma Hongli, Huang Mei, Liu Yu, Wang Jingxing, Liao Pu, Zeng Peibin, He Miao, Shan Hua
The Fourth Affiliated Hospital Zhejiang University School of Medicine, Yiwu, China.
Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
PLoS One. 2020 Jul 10;15(7):e0235612. doi: 10.1371/journal.pone.0235612. eCollection 2020.
Hepatitis C virus (HCV) genotype (GT) distribution in China shows significant geographical and demographic difference. As a routinely tested virus in Chinese blood bank systems, rare molecular epidemiology research in blood donors is reported. Our purpose is to investigate the HCV GT/subtypes distribution, phylogenetic analysis and population genetics in Chinese blood donors. Anti-HCV screen positive samples and donor demographics were collected. HCV Core and E1 gene fragments were amplified by RT-PCR, followed by sequencing and phylogenetic analysis to determine HCV GTs/subtypes using MEGA 7.0. The population genetics were performed using Arlequin v3.0 and Beast v1.10.4. SPSS Statistics 17.0 software was used to analyze the correlation between HCV GTs/subtypes distribution and demographic characteristics. 419 and 293 samples based on Core and E1 gene respectively were successfully amplified. HCV la, lb, 2a, 3a, 3b, 6a, 6e and 6n were found, and the corresponding proportions were 0.66% (3/455), 58.68% (267/455), 17.80% (81/455) and 5.05% (23/455), 3.52% (16/455), 12.31% (56/455), 0.88% (4/455) and 0.66% (3/455). Samples from Guangxi showed the most abundant genetic diversity with 8 subtypes were found. The number of haplotypes in HCV-1b is higher than 2a and 6a. The negative Tajima's D and Fu's Fs values of HCV-1b, 2a and 6a suggested the population expansion of those HCV subtypes. The distribution of HCV GT showed significant statistical difference by age and ethnicity. Conclusion: An abundance of HCV genetic diversity was found in Chinese blood donors with mainly 1b and then 2a subtype. There were significant geographical and demographic differences in HCV GTs/subtypes among Chinese blood donors. HCV subtype 1b has stronger viability and HCV subtype 6a has experienced significant expansion.
中国丙型肝炎病毒（HCV）基因型（GT）分布存在显著的地理和人口统计学差异。作为中国血库系统中的常规检测病毒，关于献血者的罕见分子流行病学研究已有报道。我们的目的是调查中国献血者中HCV GT/亚型的分布、系统发育分析和群体遗传学。收集抗-HCV筛查阳性样本和献血者人口统计学数据。通过逆转录聚合酶链反应（RT-PCR）扩增HCV核心和E1基因片段，随后进行测序和系统发育分析，使用MEGA 7.0确定HCV GT/亚型。使用Arlequin v3.0和Beast v1.10.4进行群体遗传学分析。使用SPSS Statistics 17.0软件分析HCV GT/亚型分布与人口统计学特征之间的相关性。分别基于核心基因和E1基因成功扩增了419个和293个样本。发现了HCV 1a、1b、2a、3a、3b、6a、6e和6n，相应比例分别为0.66%（3/455）、58.68%（267/455）、17.80%（81/455）、5.05%（23/455）、3.52%（16/455）、12.31%（56/455）、0.88%（4/455）和0.66%（3/455）。来自广西的样本显示出最丰富的遗传多样性，发现了8种亚型。HCV-1b的单倍型数量高于2a和6a。HCV-1b、2a和6a的Tajima's D和Fu's Fs值为负，表明这些HCV亚型的群体扩张。HCV GT的分布在年龄和种族方面存在显著统计学差异。结论：在中国献血者中发现了丰富的HCV遗传多样性，主要为1b型，其次为2a型。中国献血者中HCV GT/亚型存在显著的地理和人口统计学差异。HCV 1b亚型具有更强的生存能力，HCV 6a亚型经历了显著扩张。
