Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
FEBS Lett. 2020 Oct;594(19):3086-3094. doi: 10.1002/1873-3468.13884. Epub 2020 Aug 2.
The Golgi is surrounded by a ribosome-excluding matrix. Recently, we reported that the cis-Golgi-localized golgin GM130 can phase-separate to form dynamic, liquid-like condensates in vitro and in vivo. Here, we show that the overexpression of each of the remaining cis (golgin160, GMAP210)- and trans (golgin97, golgin245, GCC88, GCC185)-golgins results in novel protein condensates. Focused ion beam scanning electron microscopy (FIB-SEM) images of GM130 condensates reveal a complex internal organization with branching aqueous channels. Pairs of golgins overexpressed in the same cell form distinct juxtaposed condensates. These findings support the hypothesis that, in addition to their established roles as vesicle tethers, phase separation may be a common feature of the golgin family that contributes to Golgi organization.
高尔基氏体被无核糖体基质所包围。最近，我们报道了位于顺面高尔基氏体的高尔基糖蛋白 130（GM130）可以体外和体内相分离形成动态的、液态的凝聚物。在这里，我们发现其余的顺面（高尔基体 160、GMAP210）和反面（高尔基体 97、高尔基体 245、GCC88、GCC185）高尔基糖蛋白的过表达都会导致新的蛋白质凝聚物的形成。高尔基糖蛋白 130 凝聚物的聚焦离子束扫描电子显微镜（FIB-SEM）图像显示出具有分支水相通道的复杂内部组织。在同一细胞中过表达的成对高尔基糖蛋白形成明显相邻的凝聚物。这些发现支持了这样一种假设，即除了它们作为囊泡连接蛋白的既定作用之外，相分离可能是高尔基家族的一个共同特征，有助于高尔基体的组织。
