Department of Physical Chemistry, Physical Pharmacy and Bioanalysis, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland.
Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łódź, Poland.
Molecules. 2020 Jul 15;25(14):3234. doi: 10.3390/molecules25143234.
(Michx.) Elliott's (chokeberry) besides anthocyanins contains significant amounts of hydroxycinnamic acids: Chlorogenic and its isomer neochlorogenic acid. They exhibit antioxidant, anti-inflammatory, antidiabetic, and antibacterial activities, thus they can have a significant impact on the health-promoting properties of . The aim of our research was to determine the changes in the content of chlorogenic acids (CGAs) and anthocyanins during fruit development and ripening, with a particular emphasis on acids. fruit samples were collected from July to October on two organic farms in Poland. The chemical composition of the extracts was determined by NMR spectroscopy and HPLC-DAD. H-NMR and HPLC data were analyzed using chemometric analysis and multivariate statistics (PCA). The results showed that the content of chlorogenic acids and anthocyanins changes during ripening and depends on the time of harvest and the region of cultivation. A correlation between the time of CGAs reduction and the appearance of anthocyanins was also noticed. The result of our research was also a database in the form of NMR parameters, which allows analysis of the metabolite profile and tracking of its changes. The H-NMR spectrum showing anthocyanin and CGA resonance can be considered the berry fingerprint.
（Michx.）Elliott 的（越桔）除了含有花青素外，还含有大量的羟基肉桂酸：绿原酸及其异构体新绿原酸。它们具有抗氧化、抗炎、抗糖尿病和抗菌活性，因此它们可以对 的保健特性产生重大影响。我们研究的目的是确定在果实发育和成熟过程中绿原酸（CGAs）和花青素含量的变化，特别关注酸。从 7 月到 10 月，我们在波兰的两个有机农场收集了 果实样本。通过 NMR 光谱和 HPLC-DAD 确定提取物的化学成分。使用化学计量分析和多元统计（PCA）对 H-NMR 和 HPLC 数据进行分析。结果表明，绿原酸和花青素的含量在成熟过程中发生变化，这取决于收获的时间和种植的地区。还注意到 CGAs 减少的时间与花青素出现之间存在相关性。我们研究的结果也是一个以 NMR 参数形式呈现的数据库，它允许对代谢物图谱进行分析并跟踪其变化。显示花青素和 CGA 共振的 H-NMR 谱可以被认为是 越桔浆果的特征指纹。
