School of Chemical, Materials, and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA 30602, USA.
School of Chemical, Materials, and Biomedical Engineering, College of Engineering, The University of Georgia, Athens, GA 30602, USA.
Curr Opin Biotechnol. 2020 Dec;66:69-77. doi: 10.1016/j.copbio.2020.06.006. Epub 2020 Jul 16.
Engineering microbial cell factories has been widely applied to produce compounds spanning from intricate natural products to bulk commodities. In each case, host robustness is essential to ensure the reliable and sustainable production of targeted metabolites. However, it can be negatively affected by metabolic burden, pathway toxicity, and harsh environment, resulting in a decreased titer and productivity. Enhanced robustness enables host to have better production performance under complicated growth circumstances. Here, we review current strategies for boosting host robustness, including metabolic balancing, genetic and phenotype stability enhancement, and tolerance engineering. In addition, we discuss the challenges and future perspectives on microbial host engineering for increased robustness.
工程微生物细胞工厂已广泛应用于生产从复杂天然产物到大宗商品的各种化合物。在每种情况下，宿主的稳健性对于确保目标代谢物的可靠和可持续生产都是至关重要的。然而，代谢负担、途径毒性和恶劣的环境会对其产生负面影响，导致产量和生产力下降。增强稳健性可以使宿主在复杂的生长环境下具有更好的生产性能。在这里，我们综述了提高宿主稳健性的现有策略，包括代谢平衡、遗传和表型稳定性增强以及耐受工程。此外，我们还讨论了微生物宿主工程在提高稳健性方面面临的挑战和未来展望。
