Clitherow Katharina H, Binaljadm Tahani M, Hansen Jens, Spain Sebastian G, Hatton Paul V, Murdoch Craig
School of Clinical Dentistry, University of Sheffield, 19 Claremont Crescent, Sheffield S10 2TA, U.K.
Afyx Therapeutics, Lergravsej 57, 2. tv, 2300 Copenhagen, Denmark.
ACS Biomater Sci Eng. 2020 Jul 13;6(7):4087-4095. doi: 10.1021/acsbiomaterials.0c00614. Epub 2020 May 20.
Oral candidiasis is a very common oral condition among susceptible individuals, with the main causative organism being the fungus . Current drug delivery systems to the oral mucosa are often ineffective because of short drug/tissue contact times as well as increased prevalence of drug-resistant strains. We evaluated the potency of saturated fatty acids as antifungal agents and investigated their delivery by novel electrospun mucoadhesive oral patches using agar disk diffusion and biofilm assays. Octanoic (C8) and nonanoic (C9) acids were the most effective at inhibiting growth on disk diffusion assays, both in solution or when released from polycaprolactone (PCL) or polyvinylpyrrolidone/RS100 (PVP/RS100) electrospun patches. In contrast, dodecanoic acid (C12) displayed the most potent antifungal activity against pre-existing biofilms in solution or when released by PCL or PVP/RS100 patches. Both free and patch-released saturated fatty acids displayed a significant toxicity to wild-type and azole-resistant strains of . These data not only provide evidence that certain saturated fatty acids have the potential to be used as antifungal agents but also demonstrate that this therapy could be delivered directly to -infected sites using electrospun mucoadhesive patches, demonstrating a potential new therapeutic approach to treat oral thrush.
口腔念珠菌病在易感个体中是一种非常常见的口腔疾病，主要致病微生物是真菌。由于药物与组织的接触时间短以及耐药菌株的患病率增加，目前用于口腔黏膜的药物递送系统往往无效。我们评估了饱和脂肪酸作为抗真菌剂的效力，并使用琼脂平板扩散法和生物膜分析法研究了新型电纺粘膜粘附口腔贴片对其的递送情况。在平板扩散试验中，辛酸（C8）和壬酸（C9）无论是在溶液中还是从聚己内酯（PCL）或聚乙烯吡咯烷酮/RS100（PVP/RS100）电纺贴片中释放时，对抑制生长都最为有效。相比之下，十二酸（C12）在溶液中或从PCL或PVP/RS100贴片中释放时，对预先存在的生物膜显示出最强的抗真菌活性。游离的和贴片释放的饱和脂肪酸对白色念珠菌的野生型和唑类耐药菌株均表现出显著的毒性。这些数据不仅提供了证据表明某些饱和脂肪酸有潜力用作抗真菌剂，而且还证明了这种疗法可以使用电纺粘膜粘附贴片直接递送至白色念珠菌感染部位，展示了一种治疗口腔念珠菌病的潜在新治疗方法。
