Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B, Copenhagen, 2200, Denmark.
Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences University of Copenhagen, Blegdamsvej 3-B, Copenhagen, 2200, Denmark.
Curr Opin Struct Biol. 2020 Dec;65:119-129. doi: 10.1016/j.sbi.2020.06.010. Epub 2020 Jul 23.
CRISPR loci and CRISPR-associated (Cas) genes encode an adaptive immune system that protects many bacterial and almost all archaea against invasive genetic elements from bacteriophages and plasmids. Several classes of CRISPR systems have been characterized, of which the type III CRISPR systems exhibit the most unique functions. Members of type III cleave both RNA and DNA not only through their corresponding effector complexes but also by CRISPR-Cas associated proteins activated by second messengers produced by those effector complexes. Furthermore, the recent discovery of second messenger degrading proteins called ring nucleases adds an extra regulatory layer to fine-tune these immunity systems. Here, we review the defense mechanisms that govern type III CRISPR interference immunity systems focusing on the structural information available.
CRISPR 基因座和 CRISPR 相关（Cas）基因编码一种适应性免疫系统，可保护许多细菌和几乎所有古菌免受噬菌体和质粒的入侵遗传元件的侵害。已经描述了几类 CRISPR 系统，其中 III 型 CRISPR 系统表现出最独特的功能。III 型的成员不仅通过其相应的效应复合物，而且通过由这些效应复合物产生的第二信使激活的 CRISPR-Cas 相关蛋白来切割 RNA 和 DNA。此外，最近发现了一种称为环核酶的第二信使降解蛋白，为精细调节这些免疫 系统增加了一个额外的调控层。在这里，我们回顾了控制 III 型 CRISPR 干扰免疫 系统的防御机制，重点介绍了可用的结构信息。
