Univ. Lille, INSERM, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, F-59000 Lille, France.
Univ. Lille, CNRS, INRAE, Centrale Lille, UMR 8207 - UMET - Unité Matériaux et Transformations, F-59000 Lille, France.
Int J Pharm. 2020 Sep 25;587:119677. doi: 10.1016/j.ijpharm.2020.119677. Epub 2020 Jul 25.
Diabetic foot infections are the most common complications requiring hospitalisation of patients with diabetes. They often result in amputation to extremities and are associated with high morbi-mortality rates, especially when bone is infected. Treatment of these complications is based on surgical procedures, nursing care and systemic antibiotic therapy for several weeks, with a significant risk of relapse. Due to low blood flow and damage caused by diabetic foot infection, blood supply is decreased, causing low antibiotic diffusion in the infected site and an increase of possible bacterial resistance, making this type of infection particularly difficult to treat. In this context, the aim of this work was to develop a medical device for local antibiotic release. The device is a lyophilized physical hydrogel, i.e a sponge based on two oppositely charged polyelectrolytes (chitosan and poly(cyclodextrin citrate)). Cyclodextrins, via inclusion complexes, increase drug bioavailability and allow an extended release. Using local release administration increases concentrations in the wound without risk of toxicity to the body and prevents the emergence of resistant bacteria. The hydrogel was characterised by rheology. After freeze-drying, a curing process was implemented. The swelling rate and cell viability were evaluated, and finally, the sponge was impregnated with a ciprofloxacin solution to evaluate its drug release profile and its antibacterial activity.
糖尿病足感染是糖尿病患者住院治疗的最常见并发症。它们通常导致四肢截肢，并伴有高死亡率和发病率，尤其是当骨头感染时。这些并发症的治疗基于手术程序、护理和全身性抗生素治疗数周，复发风险很高。由于糖尿病足感染导致的低血流量和损伤，血液供应减少，导致感染部位的抗生素扩散减少，并增加了可能的细菌耐药性，使这种类型的感染特别难以治疗。在这种情况下，这项工作的目的是开发一种用于局部抗生素释放的医疗设备。该设备是一种冻干的物理水凝胶，即基于两种带相反电荷的聚电解质（壳聚糖和聚（环糊精柠檬酸酯））的海绵。环糊精通过包合物增加药物的生物利用度，并允许延长释放。局部释放给药可增加伤口中的浓度，而不会对身体造成毒性，并防止耐药菌的出现。该水凝胶通过流变学进行了表征。冷冻干燥后，实施了固化过程。评估了溶胀率和细胞活力，最后，将海绵浸渍在环丙沙星溶液中，以评估其药物释放曲线和抗菌活性。
