Department of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.
Scientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, Jilin, China.
J Nanobiotechnology. 2020 Jul 28;18(1):106. doi: 10.1186/s12951-020-00664-9.
Topical isotretinoin is commonly used to treat acne. However, topical isotretinoin has side effects and can hardly permeate through the stratum corneum, the most important skin barrier. Therefore, this study aimed to demonstrate the efficacy of nanoparticles as stable carriers with great curative effects, low side effects, and strong transdermal ability.
In a rabbit model of hyperkeratinization, STCM-ATRA-NPs showed significant therapeutic efficacy. By contrast, negative therapeutic efficacy was observed in a golden hamster model of hyper sebum production. Scanning electron microscopy and Fourier transform infrared spectral analyses showed that nanoparticles could penetrate the stratum corneum. Western blotting demonstrated that the nanoparticles could enhance the transdermal efficacy of isotretinoin by reducing the effect of keratin and tight junction proteins. Further, nanoparticles enhanced endocytosis, thereby promoting drug penetration and absorption into the skin.
STCM-ATRA-NPs were demonstrated to control isotretinoin release, reducing its side effects, and efficiently permeating through the skin by reducing the effect of keratin and tight junction proteins and enhancing endocytosis.
局部用异维 A 酸常用于治疗痤疮。然而，局部用异维 A 酸有副作用，而且很难穿透最重要的皮肤屏障——角质层。因此，本研究旨在证明纳米粒作为稳定载体的疗效，其具有疗效好、副作用低、透皮能力强的特点。
在兔模型的过度角化中，STCM-ATRA-NPs 表现出显著的治疗效果。相比之下，在金黄地鼠皮脂过度产生模型中则观察到治疗效果不佳。扫描电子显微镜和傅里叶变换红外光谱分析表明，纳米粒可以穿透角质层。Western blot 分析表明，纳米粒可以通过减少角蛋白和紧密连接蛋白的作用来增强异维 A 酸的透皮效果。此外，纳米粒增强了内吞作用，从而促进药物渗透和吸收进入皮肤。
STCM-ATRA-NPs 被证明可以控制异维 A 酸的释放，通过减少角蛋白和紧密连接蛋白的作用以及增强内吞作用来降低其副作用，并有效地穿透皮肤。
