Leibniz Institute of Vegetable and Ornamental Crops Großbeeren (IGZ), 14979, Großbeeren, Germany.
Institute of Bio- and Geosciences IBG-2, Plant Sciences, Forschungszentrum Jülich GmbH, 52425, Jülich, Germany.
Sci Rep. 2020 Jul 28;10(1):12574. doi: 10.1038/s41598-020-68728-2.
The soil-borne pathogen Rhizoctonia solani infects a broad range of plants worldwide and is responsible for significant crop losses. Rhizoctonia solani AG3-PT attacks germinating potato sprouts underground while molecular responses during interaction are unknown. To gain insights into processes induced in the fungus especially at early stage of interaction, transcriptional activity was compared between growth of mycelium in liquid culture and the growing fungus in interaction with potato sprouts using RNA-sequencing. Genes coding for enzymes with diverse hydrolase activities were strongly differentially expressed, however with remarkably dissimilar time response. While at 3 dpi, expression of genes coding for peptidases was predominantly induced, strongest induction was found for genes encoding hydrolases acting on cell wall components at 8 dpi. Several genes with unknown function were also differentially expressed, thus assuming putative roles as effectors to support host colonization. In summary, the presented analysis characterizes the necrotrophic lifestyle of R. solani AG3-PT during early interaction with its host.
土壤病原菌立枯丝核菌（Rhizoctonia solani）感染范围广泛，对全球农作物造成严重损失。AG3-PT 型立枯丝核菌在地下攻击发芽的马铃薯幼苗，但其互作过程中的分子响应尚不清楚。为了深入了解该真菌，特别是在互作早期阶段的诱导过程，本研究采用 RNA 测序比较了菌丝在液体培养中生长和与马铃薯幼苗互作时生长的真菌之间的转录活性。具有多种水解酶活性的酶编码基因表达差异显著，但时间响应差异显著。在 3dpi 时，主要诱导表达编码肽酶的基因，而在 8dpi 时，最强诱导的是作用于细胞壁成分的水解酶编码基因。一些具有未知功能的基因也表现出差异表达，因此推测它们可能作为效应子来支持宿主定殖。总之，本研究总结了 AG3-PT 型立枯丝核菌在与宿主早期互作过程中的坏死型生活方式。
