Opt Lett. 2020 Aug 1;45(15):4124-4127. doi: 10.1364/OL.397900.
Biomechanical contrast within tissues can be assessed based on the resonant frequency probed by spectroscopic magnetomotive optical coherence elastography (MM-OCE). However, to date, in vivo MM-OCE imaging has not been achieved, mainly due to the constraints on imaging speed. Previously, spatially-resolved spectroscopic contrast was achieved in a "multiple-excitation, multiple-acquisition" manner, where seconds of coil cooling time set between consecutive imaging frames lead to total acquisition times of tens of minutes. Here, we demonstrate an improved data acquisition speed by providing a single chirped force excitation prior to magnetomotion imaging with a BM-scan configuration. In addition, elastogram reconstruction was accelerated by exploiting the parallel computing capability of a graphics processing unit (GPU). The accelerated MM-OCE platform achieved data acquisition in 2.9 s and post-processing in 0.6 s for a 2048-frame BM-mode stack. In addition, the elasticity sensing functionality was validated on tissue-mimicking phantoms with high spatial resolution. For the first time, to the best of our knowledge, MM-OCE images were acquired from the skin of a living mouse, demonstrating its feasibility for in vivo imaging.
基于光谱磁激励光相干弹性成像（MM-OCE）探测的共振频率，可对组织的力学特性进行对比分析。然而，到目前为止，活体 MM-OCE 成像尚未实现，主要是因为受到成像速度的限制。在此之前，通过“多次激发，多次采集”的方式实现了空间分辨光谱对比度，连续成像帧之间需要几秒钟的线圈冷却时间，导致总的采集时间长达数十分钟。在此，我们通过在 BM 扫描模式下提供单次啁啾力激励，从而提高了数据采集速度。此外，还利用图形处理单元（GPU）的并行计算能力来加速弹性图的重建。加速后的 MM-OCE 平台在 2048 帧 BM 模式堆栈上实现了 2.9 s 的数据采集和 0.6 s 的后处理。此外，还在具有高空间分辨率的组织模拟体模上验证了弹性传感功能。据我们所知，这是首次从活体小鼠的皮肤中获得 MM-OCE 图像，证明了其在活体成像中的可行性。
