Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DE, United Kingdom.
Acta Crystallogr D Struct Biol. 2020 Aug 1;76(Pt 8):790-801. doi: 10.1107/S2059798320009109. Epub 2020 Jul 28.
In this article, a new approach to experimental phasing for macromolecular crystallography (MX) at synchrotrons is introduced and described for the first time. It makes use of automated robotics applied to a multi-crystal framework in which human intervention is reduced to a minimum. Hundreds of samples are automatically soaked in heavy-atom solutions, using a Labcyte Inc. Echo 550 Liquid Handler, in a highly controlled and optimized fashion in order to generate derivatized and isomorphous crystals. Partial data sets obtained on MX beamlines using an in situ setup for data collection are processed with the aim of producing good-quality anomalous signal leading to successful experimental phasing.
本文首次介绍并描述了一种用于同步加速器的大分子晶体学（MX）实验相分析的新方法。它利用自动化机器人应用于多晶体框架，将人为干预降至最低。使用 Labcyte Inc. 的 Echo 550 液体处理机，以高度可控和优化的方式自动将数百个样本浸泡在重原子溶液中，以生成衍生和同晶的晶体。使用原位数据收集设置在 MX 光束线上获得的部分数据集，通过处理旨在产生高质量的异常信号，以成功进行实验相分析。
