State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, PR China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, PR China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, PR China; Collaborative Innovation Center of Food Safety and Quality Control of Jiangsu Province, Jiangnan University, Wuxi, 214122, China.
Anal Biochem. 2020 Nov 1;608:113844. doi: 10.1016/j.ab.2020.113844. Epub 2020 Aug 4.
In this work, a colorimetric aptamer-based method for detection of cadmium using gold nanoparticles modified MoS nanocomposites as enzyme mimic is established. In short, biotinylated Cd aptamers are immobilized by biotin-avidin binding on the bottoms of the microplate, the complementary strands of Cd aptamers are connected to the Au-MoS nanocomposites which have the function of enhanced peroxidase-like activity. The csDNA-Au-MoS signal probe and target Cd compete for binding Cd aptamer, the color change can be observed by addition of chromogenic substrate, thereby realizing visual detection of Cd. The absorbance of the solution at 450 nm has a clear linear relationship with the Cd concentration. The linear range is 1-500 ng/mL, and the limit of detection is 0.7 ng/mL. The assay was used to test white wine samples, the results are consistent with those of atomic absorption spectrometry; which prove that this method can be used for detection of Cd in real samples.
在这项工作中，建立了一种基于比色适体的方法，用于检测金纳米粒子修饰的 MoS 纳米复合材料作为酶模拟物的镉。简而言之，生物素化的 Cd 适体通过生物素-亲和素结合固定在微孔板底部，Cd 适体的互补链连接到具有增强过氧化物酶样活性的 Au-MoS 纳米复合材料上。csDNA-Au-MoS 信号探针和目标 Cd 竞争与 Cd 适体结合，通过加入显色底物可以观察到颜色变化，从而实现对 Cd 的可视化检测。溶液在 450nm 处的吸光度与 Cd 浓度呈明显的线性关系。线性范围为 1-500ng/mL，检测限为 0.7ng/mL。该测定法用于测试白葡萄酒样品，结果与原子吸收光谱法一致；这证明该方法可用于检测实际样品中的 Cd。
