Sharifian Parastoo, Yaslianifard Somayeh, Fallah Parviz, Aynesazi Siavash, Bakhtiyari Mahmood, Mohammadzadeh Mohammad
Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Department of Microbiology, School of Medicine, Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran.
Infect Drug Resist. 2020 Jul 21;13:2477-2484. doi: 10.2147/IDR.S263387. eCollection 2020.
is an opportunistic pathogen that causes serious nosocomial infections, especially in immunodeficient patients and cystic fibrosis, cancer, and burned individuals. The biofilm that plays an important role in the virulence of is under the regulation of quorum sensing and two-component regulatory systems of bacteria. Curcumin, an active phenolic extract of turmeric has shown an inhibitory effect on the biofilm formation of some pathogenic bacteria. Thus, the present study aims to evaluate the effect of Nano-Curcumin on the expression of major regulatory genes involved in biofilm formation of
The biofilm formation of ATCC 10145 was assessed in the presence of 15, 20, and 25 µg/mL concentrations of Nano-Curcumin using the microplate titer method. The effect of Nano-Curcumin on the expression level of regulatory genes were determined by relative reverse transcriptase-realtime PCR.
In the absence of Nano-Curcumin, strain ATCC 10145 strongly produced biofilm (3+) and in the presence of 15 and 20 µg/mL, biofilm formation was reduced to moderate (2+) and weak biofilm producer (1+), respectively. Nano-Curcumin at a concentration of 25µg/mL inhibited biofilm formation in . The expression of regulatory genes was not affected by biofilm inhibitory concentrations of Nano-Curcumin.
The antibiofilm mechanism of Curcumin is not related to the downregulation of regulatory systems of and probably it prevents the formation of a complete biofilm structure.
[病原体名称]是一种机会致病菌，可引起严重的医院感染，尤其是在免疫缺陷患者以及患有囊性纤维化、癌症和烧伤的个体中。在[病原体名称]毒力中起重要作用的生物膜受细菌群体感应和双组分调节系统的调控。姜黄素是姜黄的一种活性酚类提取物，已显示出对某些病原菌生物膜形成的抑制作用。因此，本研究旨在评估纳米姜黄素对参与[病原体名称]生物膜形成的主要调节基因表达的影响。
使用微孔板比浊法在15、20和25μg/mL浓度的纳米姜黄素存在下评估[病原体名称]ATCC 10145的生物膜形成。通过相对逆转录实时PCR确定纳米姜黄素对调节基因表达水平的影响。
在不存在纳米姜黄素的情况下，[病原体名称]菌株ATCC 10145强烈产生生物膜（3+），在15和20μg/mL存在下，生物膜形成分别降至中度（2+）和弱生物膜产生菌（1+）。浓度为25μg/mL的纳米姜黄素抑制了[病原体名称]中的生物膜形成。纳米姜黄素的生物膜抑制浓度对调节基因的表达没有影响。
姜黄素的抗生物膜机制与[病原体名称]调节系统的下调无关，可能是它阻止了完整生物膜结构的形成。
