Investigating the Effect of Nano-Curcumin on the Expression of Biofilm Regulatory Genes of .

BACKGROUND

is an opportunistic pathogen that causes serious nosocomial infections, especially in immunodeficient patients and cystic fibrosis, cancer, and burned individuals. The biofilm that plays an important role in the virulence of is under the regulation of quorum sensing and two-component regulatory systems of bacteria. Curcumin, an active phenolic extract of turmeric has shown an inhibitory effect on the biofilm formation of some pathogenic bacteria. Thus, the present study aims to evaluate the effect of Nano-Curcumin on the expression of major regulatory genes involved in biofilm formation of

MATERIALS AND METHODS

The biofilm formation of ATCC 10145 was assessed in the presence of 15, 20, and 25 µg/mL concentrations of Nano-Curcumin using the microplate titer method. The effect of Nano-Curcumin on the expression level of regulatory genes were determined by relative reverse transcriptase-realtime PCR.

RESULTS

In the absence of Nano-Curcumin, strain ATCC 10145 strongly produced biofilm (3+) and in the presence of 15 and 20 µg/mL, biofilm formation was reduced to moderate (2+) and weak biofilm producer (1+), respectively. Nano-Curcumin at a concentration of 25µg/mL inhibited biofilm formation in . The expression of regulatory genes was not affected by biofilm inhibitory concentrations of Nano-Curcumin.

CONCLUSION

The antibiofilm mechanism of Curcumin is not related to the downregulation of regulatory systems of and probably it prevents the formation of a complete biofilm structure.