Jongeneelen F J, vd Akker W, Bos R P, Anzion R B, Theuws J L, Roelofs H M, Henderson P T
Department of Toxicology, Faculty of Medicine, University of Nijmegen, The Netherlands.
Mutat Res. 1988 Feb;204(2):195-201. doi: 10.1016/0165-1218(88)90089-4.
Liver S9 fractions were prepared from male Wistar rats, either non-induced or induced with Aroclor 1254 and from 5 human kidney transplant donors. The preparations were compared for their ability to metabolize the premutagens present in coal tar to mutagenic metabolites in the Salmonella mutagenicity assay towards strain TA98. Low levels of mutagenicity of coal tar were seen with human S9 preparations. The differences between the S9 mix of the 5 donors in capacity to activate premutagens were approximately 6-fold. The activation of coal tar by rat liver S9 preparations was higher than by the human S9 preparations. The metabolic conversion of pyrene in coal tar to 1-hydroxypyrene by the same human S9 preparations was determined in a parallel assay. 3 human preparations showed a high correlation between the formation of 1-hydroxypyrene and bioactivation of coal tar to mutagenic metabolites. The slope values of the individual regression lines were equal, suggesting that 1-hydroxypyrene is a good indicator for the activation of premutagens present in coal tar.
肝S9组分取自雄性Wistar大鼠，这些大鼠要么未用多氯联苯混合物1254诱导，要么用其诱导，同时还取自5名人类肾移植供体。在沙门氏菌致突变试验中，针对TA98菌株，比较了这些制剂将煤焦油中存在的前诱变剂代谢为诱变性代谢物的能力。用人的S9制剂观察到煤焦油的诱变性水平较低。5名供体的S9混合物在激活前诱变剂能力方面的差异约为6倍。大鼠肝脏S9制剂对煤焦油的激活作用高于人S9制剂。在平行试验中测定了相同人S9制剂将煤焦油中的芘代谢转化为1-羟基芘的情况。3种人制剂显示1-羟基芘的形成与煤焦油生物激活为诱变性代谢物之间具有高度相关性。各条回归线的斜率值相等，表明1-羟基芘是煤焦油中存在的前诱变剂激活的良好指标。
