Lee H, Lin J Y
Department of Biochemistry, Chung Shan Medical and Dental College, Taichung, Taiwan, Republic of China.
Mutat Res. 1988 Feb;204(2):229-34. doi: 10.1016/0165-1218(88)90093-6.
The antimutagenic activities of extracts of 36 commonly used anticancer crude drugs from Chinese herbs were studied by using the Salmonella/microsomal system in the presence of picrolonic acid or benzo[a]pyrene to test whether they contain direct or indirect antimutagens. Each crude drug was extracted with boiling water for 2 h, the method which is commonly used by Chinese people to prepare the drug for oral intake. The extracts of Pteris multifida P. showed the highest antimutagenic activity against picrolonic acid-induced mutation. The extracts of 6 other different kinds of Chinese herbs were shown to have a moderate antimutagenic activity against picrolonic acid-induced mutation, and they are: Actinidia chinensis P., Artemisia lavendulaefolia DC. and Crotalaria sessiflora L., Prunella vulgaris L., Paris polyphylla S. and Ampelopsis brevipedunculata T. The extracts of Smilax china L., Prunella vulgaris L. and Actinidia chinensis P. were demonstrated to inhibit the mutagenicity of benzo[a]pyrene completely. The 12 other kinds of extracts of Chinese herbs which had a moderate antimutagenic activity against benzo[a]pyrene were: Pteris polyphylla S., Ampelopsis brevipedunculata T., Duchesnea indica F., Gossypium herbaceum L., Lithospermum erythrorrhizon SZ., Artemisia lavendulaefolia DC., Selaginella doederleinii H., Dianthus superbus L., Centipeda minima ABA., Curcuma zedoaria R., Marsdenia tenacissima WA. and Kalopanax septemlobus K. Among them, there were 5 kinds of crude drugs, Actinidia chinensis P., Artemisia lavendulaefolia DC., Prunella vulgaris L., Paris polyphylla S. and Ampelopsis brevipedunculata T., containing antimutagenic factors against both picrolonic acid- and benzo[a]pyrene-induced mutation.
采用沙门氏菌/微粒体系统，在苦基酸或苯并[a]芘存在的情况下，研究了36种常用抗癌中草药粗提物的抗诱变活性，以检测它们是否含有直接或间接抗诱变剂。每种粗药均用沸水提取2小时，这是中国人常用的口服药物制备方法。凤尾草提取物对苦基酸诱导的突变显示出最高的抗诱变活性。其他6种不同中草药的提取物对苦基酸诱导的突变表现出中等抗诱变活性，它们是：中华猕猴桃、野艾蒿、猪屎豆、夏枯草、七叶一枝花和白蔹。菝葜、夏枯草和中华猕猴桃的提取物被证明能完全抑制苯并[a]芘的诱变性。其他12种对苯并[a]芘具有中等抗诱变活性的中草药提取物是：多叶凤尾蕨、白蔹、蛇莓、陆地棉、紫草、野艾蒿、江南卷柏、瞿麦、石胡荽、莪术、通光散和刺楸。其中，中华猕猴桃、野艾蒿、夏枯草、七叶一枝花和白蔹这5种粗药含有针对苦基酸和苯并[a]芘诱导突变的抗诱变因子。
