School of Pharmacy & Minhang Hospital, Fudan University, Shanghai 201301, China.
Anal Chem. 2020 Sep 15;92(18):12613-12621. doi: 10.1021/acs.analchem.0c02670. Epub 2020 Aug 26.
Senescence-associated diseases have severely diminished the quality of life and health of patients. However, a sensitive assay of these diseases remains limited due to a lack of straightforward methods. Considering that senescence-associated β-galactosidase (SA-β-Gal) is overexpressed in senescent cells, the detection of SA-β-Gal in senescent cells and tissues might be a feasible strategy for the early diagnosis of SA diseases. In this study, a β-galactosidase-activatable nanoprobe was developed for the imaging of senescent cells and vasculature in atherosclerotic mice via real-time monitoring of β-Gal. was fabricated by encapsulating a newly designed NIR ratiometric probe within a poly(lactic--glycolic) acid (PLGA) core. Nanoprobe showed good accumulation in arteries, thus successfully visualizing senescent cells and vasculature in atherosclerotic mice by tail vein injection. Our findings indicated that nanoprobe holds great potential for the early diagnosis and therapy of atherosclerosis and other aging-associated diseases.
衰老相关疾病严重降低了患者的生活质量和健康水平。然而，由于缺乏直接的方法，这些疾病的敏感检测仍然受到限制。鉴于衰老相关的β-半乳糖苷酶（SA-β-Gal）在衰老细胞中过度表达，因此检测衰老细胞和组织中的 SA-β-Gal 可能是 SA 疾病早期诊断的一种可行策略。在这项研究中，开发了一种β-半乳糖苷酶激活型纳米探针，通过实时监测β-Gal，用于成像动脉粥样硬化小鼠中的衰老细胞和血管。该纳米探针通过将新设计的近红外比率探针封装在聚乳酸-乙醇酸（PLGA）核内来制备。纳米探针在动脉中有很好的积累，因此通过尾静脉注射成功地在动脉粥样硬化小鼠中可视化衰老细胞和血管。我们的研究结果表明，纳米探针在动脉粥样硬化和其他与衰老相关的疾病的早期诊断和治疗方面具有很大的潜力。
