Department of Science and Engineering, Hamad Bin Khalifa University, Qatar Foundation, Education City, Doha, Qatar.
Department of Medical Genetics, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Mol Biol Rep. 2020 Sep;47(9):7229-7251. doi: 10.1007/s11033-020-05715-w. Epub 2020 Aug 12.
Lung cancer (LC) is among the leading causes of death all over the world and it is often diagnosed at advanced or metastatic stages. Exosomes, derived from circulating vesicles that are released from the multivesicular body, can be utilized for diagnosis and also the prognosis of LC at early stages. Exosomal proteins, RNAs, and DNAs can help to better discern the prognostic and diagnostic features of LC. To our knowledge, there are various reviews on LC and the contribution of exosomes, but none of them are about the exome techniques and also their efficiency in LC. To fill this gap, in this review, we summarize the recent investigations regarding isolation and also the characterization of exosomes of LC cells. Furthermore, we discuss the noncoding RNAs as biomarkers and their applications in the diagnosis and prognosis of LC. Finally, we compare the efficacy of exosome isolation methods to better fi + 6 + guring out feasible techniques.
肺癌（LC）是全球主要死因之一，且通常在晚期或转移阶段才被诊断出来。外泌体来源于多泡体释放的循环囊泡，可用于早期 LC 的诊断和预后。外泌体中的蛋白质、RNA 和 DNA 有助于更好地区分 LC 的预后和诊断特征。据我们所知，有许多关于 LC 和外泌体贡献的综述，但没有一篇是关于外显子技术及其在 LC 中的效率的。为了填补这一空白，在本综述中，我们总结了最近关于 LC 细胞中外泌体的分离和特征的研究。此外，我们还讨论了非编码 RNA 作为生物标志物及其在 LC 诊断和预后中的应用。最后，我们比较了外泌体分离方法的效果，以更好地确定可行的技术。
