血脑屏障（BBB）的体外和体内通透性和转运测量。
Blood-Brain Barrier (BBB) Permeability and Transport Measurement In Vitro and In Vivo.
机构信息
Department of Biomedical Engineering, The City College of the City University of New York, New York, NY, USA.
Department of Physiology and Neuroscience, Zilkha Neurogenetic Institute, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
出版信息
Methods Mol Biol. 2021;2367:105-122. doi: 10.1007/7651_2020_308.
Abstract
Quantification of the blood-brain barrier (BBB) permeability and transport in brain tissue is crucial in understanding brain disorders and developing systemic and non-systemic drug delivery strategies to the brain. This chapter summarizes BBB permeability measurement in vitro (Part I) and the in vivo non-invasive methods for quantifying the BBB permeability to solutes and brain tissue transport in rat brain by employing intravital multiphoton microscopy and a curving fitting method by using an unsteady mass transfer mathematical model (Part II).
摘要
定量测定血脑屏障（BBB）的通透性和脑组织内的转运对于理解脑部疾病以及开发向脑部输送系统性和非系统性药物的策略至关重要。本章总结了 BBB 在体外的通透性测量方法（第 I 部分），以及在活体中通过使用活体多光子显微镜和使用非稳态质量传递数学模型的曲线拟合方法来定量测定溶质的 BBB 通透性和大鼠脑内的组织转运的非侵入性方法（第 II 部分）。
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